目的 觀察養(yǎng)肝益水顆粒對自發(fā)性高血壓大鼠腎組織轉(zhuǎn)化生長因子-β₁（TGF-β₁）、磷脂酰肌醇-3激酶（PI₃K）和蛋白激酶B（PKB）的影響，探討?zhàn)B肝益水顆粒對原發(fā)性高血壓早期腎損害的作用及機制。方法 取50只12周齡收縮壓大于150 mmHg的自發(fā)性高血壓大鼠，隨機分為模型組，養(yǎng)肝益水顆粒高、中、低劑量組（10.8、5.4、2.7 g/kg）和陽性對照藥厄貝沙坦組（0.015 g/kg），每組10只；另取10只Wistar雄性大鼠作為正常對照組，模型組和正常對照組ig給予等量蒸餾水，每天1次，共12周。給藥前和給藥12周后用酶聯(lián)免疫吸附法（ELISA）檢測各組大鼠尿微量白蛋白（UMA）濃度；末次給藥后，用免疫組化法檢測各組大鼠腎臟組織中TGF-β₁和PI₃K的表達；用ELISA法檢測PKB的濃度。結(jié)果 養(yǎng)肝益水顆粒有效地降低了UMA濃度和腎臟組織TGF-β₁；升高了腎臟組織PI₃K和PKB水平。結(jié)論 養(yǎng)肝益水顆粒對高血壓的早期腎損害有一定的抑制作用，其作用機制可能與影響TGF-β₁、PI₃K和PKB的含量有關(guān)。

Objective To observe the effects of Yanggan Yishui Granule (YYG) on transforming growth factor-β₁ (TGF-β₁), phosphatidylinositol-3-kinase (PI₃K), and protein kinase B (PKB) in spontaneously hypertensive rats (SHR), and to explore the underlying mechanisms of YYG in preventing and treating early renal damage. Methods Fifty 12 weeks-old male SHR with the systolic blood pressure > 150 mmHg were randomly divided into the model group, the high-, mid-, and low-dose (10.8, 5.4, and 2.7 g/kg) YYG groups, and the positive control (Irbesartan 15 mg/kg ) group, 10 SHR in each group. Another ten Wistar rats were included as the control group. Rats in model and control groups were given the same volume of distilled water once daily for 12 weeks. At pre-administration and after successive administration for 12 weeks, urine microalbumin (UMA) concentration was measured by ELISA; after the last administration, the levels of TGF-β1 and PI₃K in liver tissue were determined by immunohistochemistry and PKB was detected by ELISA. Results YYG decreased UMA concentration and TGF-β₁ in kidney, and increased PI₃K and PKB levels in kidney. Conclusion YYG inhibites the early renal damage of SHR which might be related to its regulation on TGF-β₁, PI₃K, and PKB contents.

康緣中醫(yī)藥科技創(chuàng)新基金項目（2010HZ020）