目的 探討止顫膠囊對(duì)帕金森?。≒D）大鼠細(xì)胞因子IL-1β和IL-6表達(dá)的影響。方法 采用立體定位法將6-羥多巴胺（6-hydroxy dopamine，6-OHDA）注入大鼠黑質(zhì)部位，制備帕金森大鼠模型。實(shí)驗(yàn)分3組，包括正常對(duì)照組、模型對(duì)照組和止顫膠囊組。采用酶聯(lián)免疫吸附試驗(yàn)（ELISA）法對(duì)實(shí)驗(yàn)大鼠血清中細(xì)胞因子IL-1β和IL-6進(jìn)行分析。結(jié)果 與正常對(duì)照組相比，止顫膠囊組血清中IL-1β、IL-6基因的表達(dá)差異無顯著性。與PD模型對(duì)照組相比，止顫膠囊治療組IL-1β和IL-6的表達(dá)均有降低。結(jié)論 止顫膠囊對(duì)PD大鼠血液中IL-1β和IL-6的表達(dá)有下調(diào)作用，表明止顫膠囊對(duì)PD有一定的治療作用。

Abstract:Objective To study the effect of tremor-stopping capsule on cytokines IL-1β, IL-6 in the pathogenesis ofrats with Parkinson disease(PD). Methods Using the method of injecting 6 - hydroxydopamine (6-OHDA) ininto the substantia nigra pars of rat’s brain located to get injured PD model in part. We select. The experiment deviced into 3 groups, normal SD rats as the experimental control group, and PD rats are divided into PD model control group and tremor-stopping capsule group. ELISA were used to detect the groups all the above to analyse the expressionlevels of IL-1β and IL-6. in serum of experimental rats. The results of each group were statistically analyzed by SPSS11.5, the groups are compared with each other to determine the effects of tremor-stopping capsule on the expression of IL-1β and IL-6. Results At the beginning of experiment, IL-1β and IL-6 protein expression waslevels ware observed higher in the peripheral blood of PD model control group and tremor-stopping capsule group compared with the normal control subjects (P < 0.05); The expressionthe contents of IL-1β and IL-6 in tremor-stopping capsule group become lower followed with the time (P < 0.05), and closer to the normal control group; Those in PD model control group decreased slightly compared with those without drug treatment (P > 0.05). Conclusions The expression level of IL-1β and IL-6 in the substantia nigra parsblood of SD’s PD rats is increased caused by PD, and it is decreased of both of IL-1β and IL-6 in serum of rats who treated by the tremor-stopping capsule. It is indicated that the traditional Chinese medicine tremor-stopping capsule has some treatment on PD’s rats