目的 建立HPLC法測(cè)定忍冬藤中4種環(huán)烯醚萜苷的方法。方法 使用Diamonsil C₁₈色譜柱（250 mm×4.6 mm，5 μm），乙腈（A）-0.05%磷酸水（B）梯度洗脫，0～10 min，10%～14% A；10～35 min，14% A；體積流量1.0 mL/min，檢測(cè)波長(zhǎng)236 nm。柱溫30 ℃。結(jié)果 裂環(huán)馬錢子苷酸、馬錢苷、當(dāng)藥苷、secologanoside-7-methyl ester分別在0.806 4～32.256 μg、2.136～85.44 μg、0.793 6～31.744 μg、0.782 4～31.296 μg呈良好線性關(guān)系；回收率分別為101.27%、98.50%、101.34%、101.36%，RSD分別為2.30%、1.54%、2.10%、1.87%；結(jié)論 所建立的方法簡(jiǎn)便、準(zhǔn)確、重復(fù)性好，可用于同時(shí)測(cè)定忍冬藤中4種環(huán)烯醚萜苷的量。

Objective To establish an HPLC method for the determination of four iridoid glycosides in Lonicera Japonicae Caulis . Methods The Diamonsil C₁₈ column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase was acetonitrile (A)-0.05% phosphoric acid (B) gradient elution: 0—10 min, 10%—14% A; 10—35 min, 14% A; flow rate of 1.0 mL/min, detection wavelength at 236 nm, column temperature of 30 ℃. Results The secologanic acid, loganin, sweroside, and secologanoside-7-methyl ester presented the good linear relationship in 0.806 4—32.256, 2.136—85.44, 0.793 6—31.744, and 0.782 4—31.296 μg, respectively. Recoveries were 101.27%, 98.50%, 101.34%, and 101.36% with RSD values of 2.30%, 1.54%, 2.10%, and 1.87%. Conclusion The established method is simple, accurate, and reproducible and could be used for the simultaneous determination of four iridoid glycosides in Lonicera Japonicae Caulis.

天津市自然科學(xué)基金（11zcdzzc21000）