目的 利用⁶⁰Co-γ射線(xiàn)輻照對(duì)電子自旋共振（ESR）波譜特性的影響，建立簡(jiǎn)便、快捷的輻照中藥顆粒制劑檢測(cè)方法。方法 2種中成藥顆粒（婦樂(lè)顆粒和抗感解毒顆粒）樣品分別經(jīng)劑量率為1.0 kGy/h，劑量為0、0.1、0.3、0.7、1、3、5、7、9 kGy的⁶⁰Co-γ射線(xiàn)輻照處理，用順磁共振波譜檢測(cè)儀對(duì)樣品ESR波譜特性進(jìn)行檢測(cè)，樣品量為0.05 g，每個(gè)樣品3次重復(fù)。結(jié)果 輻照前后ESR波譜峰值有明顯差異，ESR信號(hào)強(qiáng)度隨輻照樣品吸收劑量的增加而增強(qiáng)；樣品在室溫下保存120 d，ESR信號(hào)強(qiáng)度隨時(shí)間雖有變化，但仍處于與對(duì)照組呈顯著差異的水平。結(jié)論 利用ESR波譜法可簡(jiǎn)便、快捷、有效地鑒別中藥顆粒制劑是否經(jīng)輻照處理。

Objective To develop a simple and convenient method to detect the granules preparation of Chinese patent medicine (GPCPM), based on the effect of ESR spectroscopy using ⁶⁰Co-γ ray irradiation. Methods Two kinds of GPCPM were irradiated by ⁶⁰Co-γ ray under nine doses (0, 0.1, 0.3, 0.7, 1, 3, 5, 7, and 9 kGy) at 1.0 kGy/h dose rate. The ESR spectroscopy was characterized by ESR detector with 0.05 g of sample and repeated for three times. Results It was shown that the ESR spectra were obviously different with or without irradiation. The intensity of ESR signal increased with the irradiation dose increasing, but decreased with the storage time expansion with a linear correlation. The intensity of ESR was weakened gradually during the storage at the room temperature for 120 d, though the ESR signal could be still detected. Conclusion The method of ESR spectroscopy is simple, rapid, and practically valuable for the identification of GPCPM irradiated or not.

浙江省科技廳“核農(nóng)學(xué)產(chǎn)業(yè)科技創(chuàng)新團(tuán)隊(duì)”（2010R50033）