目的 對db/db小鼠進行生物學特性研究，滿足糖尿病研究的需要。方法 在試驗周期內(nèi)，測定db/db小鼠的空腹血糖、體質量及攝食飲水量，并測定21周齡模型動物的糖耐量、血中胰島素、胰高血糖素、三酰甘油、總膽固醇等指標的水平，進行胰腺的免疫組化雙重染色及主要臟器的病理學檢查，并與db/m小鼠進行比較。結果 與對照組db/m小鼠比較，db/db小鼠過度肥胖，伴有高血糖、胰島素抵抗、脂質代謝紊亂，且肝臟和胰腺組織均出現(xiàn)明顯病變，胰腺免疫組化雙重染色結果與血液學測定結果一致，并可觀察到胰島中A細胞和B細胞的分布變化情況。結論 對自發(fā)性2型糖尿病模型db/db小鼠所做的相關生物學特性檢測，可供糖尿病研究參考。

Objective To meet the need of study on diabetes mellitus, we investigated the biological characteristics of db/db mice. Methods In this experiment, fasting blood glucose, body weight, food intake, and water intake were measured. Fasting blood glucose levels in oral glucose tolerance test were observed on db/db mice of 21 weeks old. Meanwhile, the mice were sacrificed for the detection of insulin, glucagon, triglyceride (TG), and total clolesterol (TC) in serum, immunohistochemical double staining in pancreas, and morphological study. All the data of db/db mice were compared with those of db/m mice in statistics. Results Compared with db/m mice, obesity, hyperinsulinemia, hyperglycemia, and lipid metabolism disorder were serious on db/db mice, following obvious pathological deterioration of liver and pancreas. In accordance with the result of blood testing, change in distribution of A cells and B cells could be observed in immunohistochemical double staining on pancreas of db/db mice. Conclusion The db/db mice could be used as appropriate disease models of type 2 diabetes mellitus, providing the relevant biological information for further study in this field.