目的 篩選荒漠植物老瓜頭全草鎮(zhèn)痛、抗炎作用的活性部位。方法 微波輔助萃取法制備老瓜頭全草70%乙醇提取物，分別用二氯甲烷、醋酸乙酯、正丁醇萃取3次；以二甲苯致小鼠耳腫脹法、角叉菜膠致小鼠足腫脹法為抗炎篩選方法；以冰醋酸扭體法、熱板法為鎮(zhèn)痛篩選方法。結(jié)果 與生理鹽水組比較，二氯甲烷層低、中、高劑量（200、400、800 mg/kg）對(duì)小鼠耳腫脹、足腫脹均有顯著抑制作用（P＜0.05，0.01），中、高劑量組腫脹抑制率與陽(yáng)性藥吲哚美辛相當(dāng)；二氯甲烷層低、中、高劑量均能顯著延長(zhǎng)小鼠痛閾值（P＜0.05，0.01），減少扭體次數(shù)（P＜0.05，P＜0.01）；醋酸乙酯層僅高劑量（800 mg/kg）有顯著鎮(zhèn)痛、抗炎活性（P＜0.05，P＜0.01）；正丁醇層無(wú)鎮(zhèn)痛、抗炎活性。結(jié)論 二氯甲烷層鎮(zhèn)痛、抗炎活性均最強(qiáng)，其活性與陽(yáng)性藥吲哚美辛相當(dāng)，為生品老瓜頭鎮(zhèn)痛、抗炎活性部位。

Objective To screen the active fractions of the analgesic and anti-inflammatory effects in the whole herb of desert plant, Cynanchum komarovii. Methods The 70% ethyl acetate extracts were prepared by microwave assisted extraction method and then were extracted by dichloromethane, ethyl acetate, and n-butyl alcohol for three times, respectively. The experiments of mice ear swelling induced by xylene and paw edema induced by carrageenan were applied to screen the anti-inflammatory effect, and the hot plate and glacial acetic acid writhing tests were adopted to observe the analgesic effect. Results Compared with the physiological saline group, the low-, mid-, and high-dose dichloromethane extracts (200, 400, and 800 mg/kg) had a significant inhibition on mice ear swelling and paw edema (P < 0.05, 0.01). The swelling inhibitory rates of mid- and high-dose dichloromethane extracts were equivalent to the positive medicine indomethacin. The dichloromethane extracts could significantly reduce the writhing times and prolong the pain threshold of mice. Only the high-dose ethyl acetate fraction had the analgesic and anti-inflammatory effects (P < 0.05, 0.01) and n-butyl alcohol extraction had no activity. Conclusion Dichloromethane fraction has the strong analgesic and anti-inflammatory effects equal to the positive medicine indomethacin, and is the active fraction in the whole herb of C. komarovii.

新世紀(jì)優(yōu)秀人才支持計(jì)劃（NCET-10-0915）；寧夏科技攻關(guān)寧陜合作項(xiàng)目；中國(guó)藥學(xué)會(huì)-施維雅西部項(xiàng)目資助