[關(guān)鍵詞]
[摘要]
目的 篩選荒漠植物老瓜頭全草鎮(zhèn)痛、抗炎作用的活性部位。方法 微波輔助萃取法制備老瓜頭全草70%乙醇提取物,分別用二氯甲烷、醋酸乙酯、正丁醇萃取3次;以二甲苯致小鼠耳腫脹法、角叉菜膠致小鼠足腫脹法為抗炎篩選方法;以冰醋酸扭體法、熱板法為鎮(zhèn)痛篩選方法。結(jié)果 與生理鹽水組比較,二氯甲烷層低、中、高劑量(200、400、800 mg/kg)對(duì)小鼠耳腫脹、足腫脹均有顯著抑制作用(P<0.05,0.01),中、高劑量組腫脹抑制率與陽(yáng)性藥吲哚美辛相當(dāng);二氯甲烷層低、中、高劑量均能顯著延長(zhǎng)小鼠痛閾值(P<0.05,0.01),減少扭體次數(shù)(P<0.05,P<0.01);醋酸乙酯層僅高劑量(800 mg/kg)有顯著鎮(zhèn)痛、抗炎活性(P<0.05,P<0.01);正丁醇層無(wú)鎮(zhèn)痛、抗炎活性。結(jié)論 二氯甲烷層鎮(zhèn)痛、抗炎活性均最強(qiáng),其活性與陽(yáng)性藥吲哚美辛相當(dāng),為生品老瓜頭鎮(zhèn)痛、抗炎活性部位。
[Key word]
[Abstract]
Objective To screen the active fractions of the analgesic and anti-inflammatory effects in the whole herb of desert plant, Cynanchum komarovii. Methods The 70% ethyl acetate extracts were prepared by microwave assisted extraction method and then were extracted by dichloromethane, ethyl acetate, and n-butyl alcohol for three times, respectively. The experiments of mice ear swelling induced by xylene and paw edema induced by carrageenan were applied to screen the anti-inflammatory effect, and the hot plate and glacial acetic acid writhing tests were adopted to observe the analgesic effect. Results Compared with the physiological saline group, the low-, mid-, and high-dose dichloromethane extracts (200, 400, and 800 mg/kg) had a significant inhibition on mice ear swelling and paw edema (P < 0.05, 0.01). The swelling inhibitory rates of mid- and high-dose dichloromethane extracts were equivalent to the positive medicine indomethacin. The dichloromethane extracts could significantly reduce the writhing times and prolong the pain threshold of mice. Only the high-dose ethyl acetate fraction had the analgesic and anti-inflammatory effects (P < 0.05, 0.01) and n-butyl alcohol extraction had no activity. Conclusion Dichloromethane fraction has the strong analgesic and anti-inflammatory effects equal to the positive medicine indomethacin, and is the active fraction in the whole herb of C. komarovii.
[中圖分類號(hào)]
[基金項(xiàng)目]
新世紀(jì)優(yōu)秀人才支持計(jì)劃(NCET-10-0915);寧夏科技攻關(guān)寧陜合作項(xiàng)目;中國(guó)藥學(xué)會(huì)-施維雅西部項(xiàng)目資助