目的 建立測(cè)定通樂(lè)顆粒中2, 3, 5, 4′-四羥基二苯乙烯-2-O-β-D-葡萄糖苷的方法。方法 采用HPLC法，色譜柱為Phenomenex C₁₈柱，檢測(cè)波長(zhǎng)為320 nm，流動(dòng)相為乙腈-水（20∶80），體積流量為1.0 mL/min。結(jié)果 2, 3, 5, 4′-四羥基二苯乙烯-2-O-β-D-葡萄糖苷在10～100 μg/mL與峰面積呈良好線性關(guān)系（r＝0.999 7），平均回收率為99.74%。結(jié)論 本方法準(zhǔn)確、簡(jiǎn)便、重復(fù)性好，可用于通樂(lè)顆粒中2, 3, 5, 4′-四羥基二苯乙烯-2-O-β-D-葡萄糖苷的含量測(cè)定。

Objective To establish an HPLC method for the determination of 2, 3, 5, 4′-tetetrahydroxy-stilbene-2-O-β-D-glucoside in Tongle Granule. Methods The content of 2, 3, 5, 4′-tetetrahydroxy-stilbene-2-O-β-D-glucoside was detected by HPLC. The separation was performed on a Phenomenex C₁₈ column with UV detection at 320 nm, the mobile phase consisted of acetonitrile-water (20 : 80), and the flow rate was 1.0 mL/min. Results The calibration curve of 2, 3, 5, 4′-tetetrahydroxy-stilbene-2-O-β-D-glucoside was linear within the range of 10—100 μg/mL (r = 0.999 7), and the average recovery was 99.74%. Conclusion The method to determine the content of 2, 3, 5, 4′-tetetrahydroxy-stilbene-2-O-β-D-glucoside is accurate, simple, and reproducible, and could be used in the quantitative analysis of 2, 3, 5, 4′-tetetrahydroxy-stilbene-2-O-β-D-glucoside in Tongle Granule.