目的 研究蛹蟲草液體發(fā)酵產(chǎn)物凍干粉的抗氧化活性及保肝作用。方法 采用真空冷凍干燥的方法將蛹蟲草液體發(fā)酵產(chǎn)物制成干粉并對凍干粉主要成分進行分析測定，通過DPPH自由基清除實驗和衰老小鼠模型驗證蛹蟲草發(fā)酵產(chǎn)物凍干粉的抗氧化活性，通過小鼠急性肝損傷實驗研究凍干粉的保肝作用，采用急性毒性試驗驗證凍干粉的安全性。結(jié)果 蛹蟲草發(fā)酵產(chǎn)物凍干粉含豐富的粗纖維、粗多糖、粗三萜及黃酮類物質(zhì)。蛹蟲草發(fā)酵產(chǎn)物凍干粉對DPPH自由基的半數(shù)有效濃度（EC₅₀）為8.12 mg/mL，在16 mg/mL時對DPPH自由基的清除率為61.56%，與0.02 mg/mL的維生素C（Vc）清除能力相當；蛹蟲草發(fā)酵產(chǎn)物能顯著提高衰老模型小鼠血清和肝臟中的總超氧化物歧化酶（T-SOD）、谷胱甘肽過氧化物酶（GSH-px）活力水平，降低丙二醛（MDA）含量，高劑量組的抗衰老效果與陽性藥Vc組的抗氧化能力相當。小鼠急性肝損傷試驗結(jié)果顯示，凍干粉能有效降低模型組小鼠血液中門冬氨酸氨基轉(zhuǎn)移酶（AST）與丙氨酸氨基轉(zhuǎn)移酶（ALT）水平（P＜0.05）。凍干粉的半數(shù)致死量（LD₅₀）遠大于國家無毒標準15 g/kg，屬于無毒級別。結(jié)論 蛹蟲草發(fā)酵產(chǎn)物具有良好的抗氧化活性和保肝作用，且安全無毒。

Objective To test the anti-oxidative activity of lyophilized powder of liquid fermentation products from Cordyceps militaris. Methods The lyophilized powder of liquid fermentation products from Cordyceps militaris was prepared by freezing and drying process, then, the anti-oxidative capacity of lyophilized powder was measured by DPPH tests in vitro and the aging mouse model was induced by D-galactose in vivo. Results The results indicated that the EC₅₀ value of the lyophilized powder on scavenging DPPH free radical activity was 8.12 mg/mL, comparing to Vitamin C (Vc), and the scavenging DPPH activity of the lyophilized powder with the concentration of 16 mg/mL was equal to that of 0.02 mg/mL Vc; The aging mice model tests indicated the lyophilized powder could effectively improve the levels of total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-px), reduce the level of malondiadehyde (MDA) in serum and liver of aging mice (P < 0.05). The acute liver injury test results showed that the lyophilized powder could effectively reduce the contents of aspartate transaminase (GOT) and glutamic-pyruvic transaminase (GPT) in serum of mice with acute liver injury (P < 0.05). Toxic tests indicated that the lyophilized belonged to the Non-toxic level. Conclusion The Cordyceps militaris liquid fermentation products as a safety food have strong anti-oxidative activity and hepatoprotective effect.

河北省科技計劃項目（11230908D）；河北省教育廳重點項目（ZH2011231）資助