目的 研究復方鴉膽子油脂質體凍干粉的性質，建立其質量評價方法。方法 考察復方鴉膽子油脂質體的形態(tài)、粒徑分布、Zeta電位，采用TLC法對其定性鑒別，并采用溶劑萃取法測定脂質體包封率。同時，通過UV和GC的方法測定其有效成分的含量。結果 復方鴉膽子油脂質體凍干粉色澤均勻，質地細膩，形態(tài)飽滿，其平均粒徑為170.3 nm，多分散系數（PDI）為0.258，Zeta電位為?32.1 mV，帶負電，脂質體pH為5.05。TLC鑒別中檢出蟾酥、鴉膽子油斑點清晰可見。脂質體凍干粉的平均包封率為91.90%，吲哚類生物堿的平均質量分數為5.19 mg/g，油酸的平均質量分數為79.08 mg/g，亞油酸的平均質量分數50.41 mg/g。脂質體的平均過氧化值為0.030 5。結論 上述方法適用于復方鴉膽子油脂質體凍干粉的質量評價，可有效反映其各方面的性質。

Objective To study the properties of liposomes freeze-powder of compound Brucea Javanica oil (LFP-CBO), and to establish the quality evaluation methods. Methods The form, particles distribution, and Zeta potential of LFP-CBO were investigated, TLC was used to identify the chemical composition, the encapsulation efficiency was determined by solvent extraction method, and the active ingredients was determined by UV and GC. Results The mean particle size of LFP-CBO was 170.3 nm with uniform color, fine texture, and round shape, and the polydisperse index (PDI) was 0.258. The Zeta potential was ?32.1 mV with negative charge, and the pH value was 5.05. The spots of B. Javanica and Veneum Bufonis were clear on TLC. The average encapsulation efficiency of the optimal formulation was 91.9%. The concentration of indole alkaloids, oleic acid, and linoleic acid was 5.19, 79.08, and 50.41 mg/g, and the peroxide value of LFP-CBO was 0.030 5. Conclusion The methods above are suitable for the quality evaluation of LFP-CBO, and could effectively demonstrate its properties.