目的 探討延齡草苷（trillin）對(duì)小膠質(zhì)細(xì)胞（BV-2）的炎性激活的抑制作用。方法 不同濃度的延齡草苷孵育0.5 h，然后用脂多糖進(jìn)行刺激，培養(yǎng)24 h后收集上清，Greiss法檢測NO濃度；CCK-8檢測延齡草苷對(duì)小膠質(zhì)細(xì)胞活力的影響；Elisa檢測腫瘤壞死因子（TNF-α）的濃度；實(shí)時(shí)定量PCR（RT-PCR）檢測TNF-α、IL-1β、IL-6、iNOS、COX-1、COX-2基因的表達(dá)。結(jié)果 在不影響細(xì)胞活力的前提下，延齡草苷能明顯抑制NO的釋放，同時(shí)能抑制激活誘導(dǎo)細(xì)胞死亡，能明顯抑制TNF-α的產(chǎn)生，能明顯抑制TNF-α、IL-1β、IL-6、iNOS、COX-2 mRNA的表達(dá)，但是不能抑制COX-1 mRNA的表達(dá)。結(jié)論 延齡草苷可以抑制LPS誘導(dǎo)的小膠質(zhì)細(xì)胞的炎性激活，抑制炎性基因的表達(dá)。

Objective To study the inhibition of trillin on the inflammatory activation of microglia (BV-2). Methods The BV-2 cells were incubated with trillin at different concentration for 0.5 h and stimulated with lipopolysaccharide (LPS). The supernatant was collected after 24 h culture, and the NO level was detected by Greiss method; The activity of BV-2 cells was detected with CCK-8; The expression of tumor necrosis factor (TNF-α), IL-1β, IL-6, iNOS, COX-1, and COX-2 was detected by real time quantitative (RT-PCR). Results Trillin can inhibit the NO release in microglia induced by LPS without affecting the cell viability and inhibit the inflammatory factors such as TNF-α, IL-1β, IL-6, iNOS, and COX-2. Conclusion Trillin can inhibit the inflammatory activationof BV-2 cells induced by LPS and inhibit the expression of inflammatory factor.

國家重大新藥創(chuàng)制項(xiàng)目（2011ZX09201-201）