目的 評價12種降壓西藥的寒熱藥性。方法 用噻唑藍(lán)（MTT）比色法檢測12種降壓西藥對HepG2細(xì)胞體外增殖的影響，并用倒置顯微鏡觀察其對細(xì)胞形態(tài)特征的影響。結(jié)果 利血平、倍他洛克、卡托普利、硝苯地平、氨氯地平的藥性為寒性；普萘洛爾、培哚普利、雷米普利、氯沙坦鉀、尼群地平、吲達(dá)帕胺、氫氯噻嗪為熱性。形態(tài)學(xué)觀察表明利血平、倍他洛克、卡托普利、硝苯地平、氨氯地平使細(xì)胞密度減小，固縮變圓；普萘洛爾、培哚普利、雷米普利、氯沙坦鉀、尼群地平、吲達(dá)帕胺、氫氯噻嗪在低濃度時可使細(xì)胞密度增加，結(jié)構(gòu)致密、生長旺盛。結(jié)論 細(xì)胞學(xué)方法能夠用于西藥寒熱藥性的評價，這為西藥中藥化提供了一種簡單實(shí)用的評價寒熱藥性的方法。

Objective To evaluate the cold-heat characteristics of 12 kinds of hypotensive drugs. Methods MTT assay was used to investigate the effect of the 12 kinds of hypotensive drugs on the growth and proliferation of cultured HepG2 cells in vitro. Morphological changes were observed through inverted microscope. Results Reserpine, Metoprolol, Captopril, Nifedipine, and AmLodipine showed cold or cool characteristics, while Propranolol, Perindopril, Ramipril, Losartan potassium, Nitrendipine, Indapamide, and Hydrochlorohthiazide showed hot or warm characteristics. Morphological observation demonstrated that the cell density of the cells treated with Reserpine, Metoprolol, Captopril, Nifedipine, and AmLodipine decreased, but the cell density of the cells treated with Propranolol, Perindopril, Ramipril, Losartan potassium, Nitrendipine, Indapamide, and Hydrochlorothiazide increased. Conclusion The cytological method could be used to evaluate the cold-heat characteristics of Western medicine. This simple and reliable method will promote the Chinese medicalization of Western medicine.

國家自然科學(xué)基金資助項(xiàng)目（20872118，30070905）；教育部博士點(diǎn)基金資助項(xiàng)目（20070697012）；陜西省重大科技專項(xiàng)資助項(xiàng)目 （2008ZDKG-67）；陜西省重點(diǎn)實(shí)驗(yàn)室基金資助項(xiàng)目（08JZ74，2010JS097，11JS090）