[關(guān)鍵詞]
[摘要]
目的 制備新型抗炎鎮(zhèn)痛化合物4-鄰甲苯磺酰氧基苯并噁唑酮(MBB)脂質(zhì)體并對(duì)其進(jìn)行質(zhì)量控制方法研究。方法 采用薄膜分散法制備MBB脂質(zhì)體,觀察脂質(zhì)體的形態(tài),測定其粒徑與電位,采用HPLC法測定其含量,并考察其包封率、滲漏率、穩(wěn)定性等。結(jié)果 制備的MBB脂質(zhì)體平均粒徑為46.3 nm,電位為-60.25 mV,包封率為94%,且于4 ℃下儲(chǔ)存較穩(wěn)定。結(jié)論 所制備的脂質(zhì)體粒徑均一,包封率高,穩(wěn)定性好,所建立的HPLC分析方法準(zhǔn)確可靠、重復(fù)性好、操作簡便、專屬性強(qiáng),符合質(zhì)量測定要求。
[Key word]
[Abstract]
Objective To prepare the liposomes of 4-O-methyl-benzenesulfonyl-2 (3H)-benzoxazolone (MBB), an anti-inflammatory agent, and to investigate the quality control methods. Methods The liposomes were prepared by the film dispersion method, then the morphology and diameter were detected. The content of MBB was determined by HPLC method, and the entrapment efficiency, percolation rate, and stability of liposomes were investigated, respectively. Results The mean diameter of the liposomes was 46.3 nm, and the potential was -60.25 mV. The encapsulation efficiency was 94%. The liposomes were stable at 4 ℃. Conclusion The prepared MBB liposomes show homogeneous diameter, high encapsulation efficiency, and good stability. The HPLC method is sensitive and accurate with good specificity which could be used for the quality control of MBB liposomes.
[中圖分類號(hào)]
[基金項(xiàng)目]
山西省科技攻關(guān)項(xiàng)目(20110321076)