目的 建立刺五加中異嗪皮啶的定量方法，并對主產(chǎn)區(qū)采集的刺五加野生藥材的不同部位、不同生長年限及不同生長環(huán)境的異嗪皮啶的量進(jìn)行測定，為分析刺五加野生藥材的質(zhì)量提供參考。方法 刺五加異嗪皮啶的提取采用超聲提取法，HPLC法測定，色譜柱為Diamonsil C₁₈（250 mm×4.6 mm，5 mm），流動相為乙腈-0.1%磷酸（20：80），檢測波長344 nm，體積流量1.0 mL/min。結(jié)果 異嗪皮啶進(jìn)樣量在16.8～252.0 ng呈現(xiàn)良好的線性關(guān)系（r=0.999 9），平均回收率為100.4%，RSD值為1.0%（n=6）。刺五加不同部位中，皮的異嗪皮啶含量最高，莖次之，根及根莖含量較低。不同生長年限中，5年生刺五加異嗪皮啶含量最高，3年生含量次之，2年以下含量最低。陽坡生長的異嗪皮啶含量高于陰坡生長的藥材?？傮w分析異嗪皮啶的含量黑龍江高于吉林。結(jié)論 本方法可操作性強(qiáng)，簡便快速，重復(fù)性好，回收率高，穩(wěn)定可靠，可作為刺五加藥材的質(zhì)控方法。鑒于野生藥材生長年限不一，分布不集中，與栽培的藥材相比含量波動大，因此，在藥品生產(chǎn)過程中，根據(jù)對中間體或成品中異嗪皮啶含量的要求，有必要建立刺五加藥材異嗪皮啶的含量標(biāo)準(zhǔn)，以保證生產(chǎn)藥品的質(zhì)量。

Objective To establish a method for the determination of isofraxidin in Acanthopanax senticosus, and the content of isofraxidin in wild A. senticosus collected from main producing areas in different parts, growth years, and growth conditions were measured. This provides a reference for quality analysis of wild A. senticosus. Methods Isofraxidin in A. senticosus was extracted using ultrasonic extraction. The HPLC analysis was carried out on a Diamonsil C₁₈ column (1) (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-0.1% phosphoric acid (20:80) and the flow rate was 1.0 mL/min. The detective wavelength was set to 344 nm. Results The calibration curve was linear with the sample load of 0.016 8—0.252 0 μg (r = 0.999 9). The average recovery was 100.4% with RSD of 1.0% (n = 6). The highest content of isofraxidin from same herb was in skin, followed by stems, and in roots and rhizomes the content was the lowest. The highest content of different ages was for five years to birth, followed by three years, and for less than two years was the lowest. The growth content of herbs in sunny area was higer than that in shady area. Based on the above analyzed, the content of isofraxidin in A. senticosus from Heilongjiang were higher than those from Jilin. Conclusion Considering about the different growth periods, distribution and fluctuation of content compared with the cultivation of medicinal materials, it is necessary to establish the content standards for isofraxidin of A. senticosus to ensure the quality of pharmaceuticals production, according to the isofraxidin content requirements of the intermediate or finished medicine. This method is simple, rapid, accurate, and replicate. And it can be adopted for the quality control of A. senticosus.

天津市重大科技專項(xiàng)（工程）項(xiàng)目：龍加通絡(luò)膠囊產(chǎn)業(yè)化研究