18(250 mm×4.6 mm,5 μm)色譜柱,以1%冰醋酸為流動(dòng)相A,乙腈為流動(dòng)相B進(jìn)行梯度洗脫,體積流量為1.0 mL/min,檢測(cè)波長(zhǎng)為230 nm。結(jié)果 毛蕊花糖苷、馬錢苷、丹皮酚和芍藥苷測(cè)定濃度的線性范圍分別為6~60、15~150、50~500 μg/mL和5~50 μg/mL,r值均大于0.999,平均加樣回收率分別為98.3%、99.3%、99.0%和99.0%,相對(duì)標(biāo)準(zhǔn)偏差(RSD)值均小于0.9%(n=9)。結(jié)論 該實(shí)驗(yàn)方法簡(jiǎn)便、結(jié)果準(zhǔn)確,可有效地用于六味地黃膠囊的質(zhì)量控制。;Objective An HPLC method was established for determining four constituents (verbascoside, loganin, paeoniflorin, and paeonolum ) in Liuwei Dihuang Capsule. Methods HPLC-gradient elution method was applied. The column was Kromasil C18 (250 mm × 4.6 mm, 5 μm). The mobile phase was 1% acetic acid solution (A) and acetonitrile (B). The detection wavelength was set at 245 nm. Results The linear range of density was 6—60 μg/mL for verbascoside, 15—150 μg/mL for loganin, 50—500 μg/mL for paeonolum, and 5—50 μg/mL for paeoniflorin, respectively. The correlation coefficient of all curves were better than 0.999. The average recoveries of four analytes were 98.3% for verbascoside, 99.3% for loganin, 99.0% for paeonolum, and 99.0% for paeoniflorin, and the RSD values were less than 0.9% (n = 9). Conclusion The developed HPLC method could be used for the quality control of Liuwei Dihuang Capsule."/>

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首頁(yè) > 過(guò)刊瀏覽>2014年第37卷第3期 >2014,37(3):257-259. DOI:10.7501/j.issn.1674-6376.2014.03.015
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HPLC法測(cè)定六味地黃膠囊中的毛蕊花糖苷、馬錢苷、芍藥苷和丹皮酚

Simultaneous determination of four constituents in Liuwei Dihuang Capsule by HPLC

發(fā)布日期:2014-06-06
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