目的 探索建立超快速液相色譜（UFLC）法測定復(fù)方丹參片中三七皂苷R₁、人參皂苷Rg₁及Rb₁。方法 采用SHIMADZU Shim-pack XR-ODS Ⅲ（2.0 mm×75 mm， 1.6 μm）色譜柱；以乙腈-水作為流動相進行梯度洗脫；體積流量為0.4 mL/min；檢測波長為203 nm；進樣體積為3 μL。結(jié)果 三七皂苷R₁、人參皂苷Rg₁及Rb₁分別在0.025 7～0.257 0、0.101 2～1.012 0、0.104 4～1.044 0 μg與峰面積呈良好的線性關(guān)系，平均加樣回收率分別為96.7%、98.1%、98.8%。結(jié)論 本方法在15min內(nèi)可以將三七皂苷R₁、人參皂苷Rg₁及Rb₁有效分離，節(jié)省了大量人力和流動相的消耗，為中藥的質(zhì)量控制技術(shù)提供參考方法。

Objective To establish the quantitative method of notoginsenoside R₁, ginsenosides Rg₁ and Rb₁ in Compound Danshen Tablet by ultra-fast liquid chromatography (UFLC). Methods This assay was performed on Shimadzu Shim-pack XR-ODS Ⅲ (75 mm × 2.0 mm, 1.6 μm) column with acetonitrile-water as mobile phase in gradient elution at a flow rate of 0.4 mL/min; And the detection wavelength was 203 nm. The injection volume was 3 μL. Results The linear of notoginsenoside R₁, ginsenosides Rg₁ and Rb₁ had good linearity within the range of 0.025 7—0.257, 0.101 2—1.012, and 0.104 4—1.044 μg. And their average recovery ratios were 96.7%, 98.1%, and 98.8%. Conclusion UFLC method may greatly improve the separation efficiency and analysis speed in the case of notoginsenoside R₁, ginsenosides Rg₁ and Rb₁ in Compound Danshen Tablet while reducing the solvent consumption. As an alternative of conventional HPLC, UPLC is more convenient, fast, and feasible.