18柱(250 mm×4.6 mm,5 μm),以甲醇-0.05%磷酸水(33:67)為流動(dòng)相,在330 nm檢測(cè)波長(zhǎng)下同時(shí)對(duì)復(fù)方利膽解毒顆粒中有效成分綠原酸和連翹酯苷A進(jìn)行定量測(cè)定。結(jié)果 綠原酸在0.044 72~0.894 4 μg線性關(guān)系良好,r=1.000 0,平均回收率為98.92%,RSD為1.56%;連翹酯苷A在0.052 16~1.043 2 μg線性關(guān)系良好,r=0.999 9;平均回收率為98.36%,RSD為1.44%。結(jié)論 本方法專屬性強(qiáng)、簡(jiǎn)便、準(zhǔn)確,可有效控制該制劑的質(zhì)量。;Objective To establish a method for detecting the quantity of chlorogenic acid and forsythoside A in Compound Lidanjiedu Granule. Methods The HPLC method was carried out using a Diamonsil-C18 column (250 mm × 4.6 mm, 5 μm) eluted with the mobile phases of methanol-0.05% phosphoric acid (33:67). The method could detect the quantity of chlorogenic acid and forsythoside A in Compound Lidanjiedu Granule simultaneously with the detective wavelength of 330 nm. Results The linear range of chlorogenic acid was 0.044 72 — 0.894 4 μg (r = 1.0000), the recovery was 98.92%. and the RSD value was 1.56%; The linear range of forsythoside A was 0.052 16 — 1.0432 μg (r = 1.0000), the recovery was 98.36%, and the RSD value was 1.44%. Conclusion The method is specific, simple, and accurate, which could control the quality of Compound Lidanjiedu Granule."/>