20H2209）的含量測(cè)定方法。方法 采用高效液相色譜（HPLC）法，以Dionex Acclaim 120® C18色譜柱（150 mm×4.6 mm，5 μm）為分離柱，以乙腈-1%甲酸溶液（23：77）為流動(dòng)相，體積流量1.0 mL/min，檢測(cè)波長(zhǎng)320 nm，柱溫25 ℃。結(jié)果 2，3，5，4’-四羥基二苯乙烯-2-O-β-D-葡萄糖苷在0.010～0.200 μg呈現(xiàn)良好的線性關(guān)系（r=0.999 6），平均回收率為97.35%，RSD為2.07%。結(jié)論 該方法<準(zhǔn)確可靠，適用于安爾眠膠囊中2，3，5，4’-四羥基二苯乙烯-2-O-β-D-葡萄糖苷的含量測(cè)定。;Objective To establish the method for the content determination of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside in Anermian Capsule by HPLC. Methods The chromatographic condition was Dionex Acclaim 120® C18 column (150 mm × 4.6 mm, 5 μm), mobile phase was acetonitrile-water (containing 1% formic acid) (23:77), flow rate was 1.0 mL/min, detective wavelength was 320 nm, and column temperature was 25 ℃. Results 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside had a good linear relationship in the range of 0.010—0.200 μg (r = 0.999 6), and the mean recovery rate was 97.35% (RSD = 2.07%). Conclusion The method is accurate and reliable for determination of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside in Anermian Capsule."/>

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首頁(yè) > 過(guò)刊瀏覽>2014年第37卷第3期 >2014,37(3):266-268. DOI:10.7501/j.issn.1674-6376.2014.03.018
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HPLC測(cè)定安爾眠膠囊中2,3,5,4’-四羥基二苯乙烯-2-O-β-D-葡萄糖苷

Determination of 2,3,5,4’- tetrahydroxystilbene-2-O-β-D-glucoside in Anermian Capsule by HPLC

發(fā)布日期：2014-06-06
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    [關(guān)鍵詞]
    [摘要]
    目的 建立安爾眠膠囊中2，3，5，4’-四羥基二苯乙烯-2-O-β-D-葡萄糖苷（C20H2209）的含量測(cè)定方法。方法 采用高效液相色譜（HPLC）法，以Dionex Acclaim 120® C18色譜柱（150 mm×4.6 mm，5 μm）為分離柱，以乙腈-1%甲酸溶液（23：77）為流動(dòng)相，體積流量1.0 mL/min，檢測(cè)波長(zhǎng)320 nm，柱溫25 ℃。結(jié)果 2，3，5，4’-四羥基二苯乙烯-2-O-β-D-葡萄糖苷在0.010～0.200 μg呈現(xiàn)良好的線性關(guān)系（r=0.999 6），平均回收率為97.35%，RSD為2.07%。結(jié)論 該方法<準(zhǔn)確可靠，適用于安爾眠膠囊中2，3，5，4’-四羥基二苯乙烯-2-O-β-D-葡萄糖苷的含量測(cè)定。
    [Key word]
    [Abstract]
    Objective To establish the method for the content determination of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside in Anermian Capsule by HPLC. Methods The chromatographic condition was Dionex Acclaim 120® C18 column (150 mm × 4.6 mm, 5 μm), mobile phase was acetonitrile-water (containing 1% formic acid) (23:77), flow rate was 1.0 mL/min, detective wavelength was 320 nm, and column temperature was 25 ℃. Results 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside had a good linear relationship in the range of 0.010—0.200 μg (r = 0.999 6), and the mean recovery rate was 97.35% (RSD = 2.07%). Conclusion The method is accurate and reliable for determination of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside in Anermian Capsule.
    [中圖分類(lèi)號(hào)]
    [基金項(xiàng)目]
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