20H2209)的含量測(cè)定方法。方法 采用高效液相色譜(HPLC)法,以Dionex Acclaim 120® C18色譜柱(150 mm×4.6 mm,5 μm)為分離柱,以乙腈-1%甲酸溶液(23:77)為流動(dòng)相,體積流量1.0 mL/min,檢測(cè)波長(zhǎng)320 nm,柱溫25 ℃。結(jié)果 2,3,5,4’-四羥基二苯乙烯-2-O-β-D-葡萄糖苷在0.010~0.200 μg呈現(xiàn)良好的線性關(guān)系(r=0.999 6),平均回收率為97.35%,RSD為2.07%。結(jié)論 該方法<準(zhǔn)確可靠,適用于安爾眠膠囊中2,3,5,4’-四羥基二苯乙烯-2-O-β-D-葡萄糖苷的含量測(cè)定。;Objective To establish the method for the content determination of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside in Anermian Capsule by HPLC. Methods The chromatographic condition was Dionex Acclaim 120® C18 column (150 mm × 4.6 mm, 5 μm), mobile phase was acetonitrile-water (containing 1% formic acid) (23:77), flow rate was 1.0 mL/min, detective wavelength was 320 nm, and column temperature was 25 ℃. Results 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside had a good linear relationship in the range of 0.010—0.200 μg (r = 0.999 6), and the mean recovery rate was 97.35% (RSD = 2.07%). Conclusion The method is accurate and reliable for determination of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside in Anermian Capsule."/>