目的 建立安爾眠膠囊中2，3，5，4’-四羥基二苯乙烯-2-O-β-D-葡萄糖苷（C₂₀H₂₂0₉）的含量測定方法。方法 采用高效液相色譜（HPLC）法，以Dionex Acclaim 120^® C₁₈色譜柱（150 mm×4.6 mm，5 μm）為分離柱，以乙腈-1%甲酸溶液（23：77）為流動相，體積流量1.0 mL/min，檢測波長320 nm，柱溫25 ℃。結(jié)果 2，3，5，4’-四羥基二苯乙烯-2-O-β-D-葡萄糖苷在0.010～0.200 μg呈現(xiàn)良好的線性關(guān)系（r=0.999 6），平均回收率為97.35%，RSD為2.07%。結(jié)論 該方法<準(zhǔn)確可靠，適用于安爾眠膠囊中2，3，5，4’-四羥基二苯乙烯-2-O-β-D-葡萄糖苷的含量測定。

Objective To establish the method for the content determination of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside in Anermian Capsule by HPLC. Methods The chromatographic condition was Dionex Acclaim 120^® C₁₈ column (150 mm × 4.6 mm, 5 μm), mobile phase was acetonitrile-water (containing 1% formic acid) (23:77), flow rate was 1.0 mL/min, detective wavelength was 320 nm, and column temperature was 25 ℃. Results 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside had a good linear relationship in the range of 0.010—0.200 μg (r = 0.999 6), and the mean recovery rate was 97.35% (RSD = 2.07%). Conclusion The method is accurate and reliable for determination of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside in Anermian Capsule.