目的 建立測定寧嗽化痰丸中橙皮苷的高效液相色譜（HPLC）法。方法 采用美國的LabAlliance儀器，色譜柱為ZORBAX Eclipse XDB-C18（150 mm × 4.6 mm，5 μm）；流動相為甲醇-0.5%醋酸（39：61）；檢測波長為284 nm，柱溫40 ℃；體積流量0.8 mL/min。理論板數(shù)按橙皮苷計算應不低于3 000。結(jié)果 橙皮苷線性范圍為0.020 9～0.209 0 mg/mL，平均加樣回收率為99.86%，RSD值為1.88%。結(jié)論 該方法簡單、快速、準確，可作為寧嗽化痰丸質(zhì)量控制方法。

Objective To extablish an HPLC method to determine the content of hesperidin in Ningsou Huatan Pill. Methods The content of hesperidin in NingSou Huatan Pill was determined by HPCL method. The LabAlliance Apparatus from US was used, octadecyl silica bonded silica gel was used as packing agent; The mobile phase was methanol-0.5% acetic acid (39:61), with the wavelength of 284 nm, column temperature of 40 ℃, and flow rate of 0.8 mL/min. The number of theoretical plates was no lower than 3 000 in terms of hesperidin. Results The linear scope of hesperidin was 0.0209—0.2090 mg/mL, sample recovery rate in average was 99.86%, and the RSD value was 1.88%. Conclusion The method with HPLC is simple, rapid, and accurate, thus can be used as quality control method of Ningsou Huatan Pill.