18(250 mm×4.6 mm,5 μm),以乙腈-0.05%磷酸溶液進行梯度洗脫,柱溫為30 ℃,體積流量為0.8mL/min,檢測波長為220 nm。結果 初步建立了天麻配方顆粒的高效液相色譜指紋圖譜,確定了6 個特征峰。結論 高效液相色譜指紋圖譜可為天麻配方顆粒的鑒別和質(zhì)量控制提供參考。;Objective To establish the HPLC characteristic chromatogram of Formula Granule of Gastrodiae Rhizoma as a reference for the identification and quality control of this formula granule. Methods The chromatograms were obtained using the Megres5-C18 column (250 mm × 4.6 mm, 5 μm) with mobile phase comprised of acetonitrile-0.05% phosphoric acid aqueous solution in gradient elution at flow rate of 0.8 mL/min, column temperature 30 ℃, detection wavelength 220 nm and sample size of 10 μL. Results The HPLC characteristic chromatogram of Formula Granule of Gastrodiae Rhizoma consisted of 6 common peaks. The similarity of 10 batches of samples was above 0.9. Conclusion The HPLC method is proved to be accurate, stable and reproducible and can provide reference for the identification and quality control of Formula Granule of Gastrodiae Rhizoma."/>