目的 建立一種測(cè)定二母安嗽片中嗎啡的方法。方法 采用反相高效液相色譜法測(cè)定二母安嗽片中嗎啡的量。色譜柱為phenomenex Luna C₁₈（2）（250 mm×4.6 mm，5 μm），流動(dòng)相為乙腈-0.1%磷酸溶液（15：85）（每100 mL 加0.1 g 庚烷磺酸鈉），柱溫40 ℃，體積流量1.0 mL/min，檢測(cè)波長(zhǎng)220 nm。結(jié)果 嗎啡在3.907～97.680 μg/mL 與峰面積積分值呈良好線性關(guān)系，平均回收率為96.78%，RSD=0.64%（n=6）。結(jié)論 所建立的方法操作簡(jiǎn)便、測(cè)定準(zhǔn)確、重復(fù)性良好，可用于二母安嗽片的質(zhì)量控制。

Objective To establish a method for the determination of morphine in Ermu Ansou Tablet. Methods RP-HPLC was applied to determine the content of morphine. An RP-HPLC method was established with Luna C₁₈ column (2) (250 mm × 4.6 mm, 5 μm) by a gradient elution using acetonitrile-0.1% phosphoric acid (15:85) (0.1 g sodium heptanesulfonate added into 100 mL); The flow rate was 1.0 mL/min and the wavelength was set at 220 nm. The column temperature was 40 ℃. Results The relationship between injection level and area of morphine was evaluated to be linear at the range of 3.907—97.680 μg/mL. The average recovery rate was 96.78%, and RSD was 0.64% (n = 6). Conclusion The quantitative methods established in this study are convenient, accurate, and reproducible, and can be used as the quality control standard for Ermu Ansou Tablet.