目的 探討毒蕈堿(M)受體及其亞型對(duì)四磨湯(SMD)誘發(fā)胃竇縱、環(huán)行肌收縮的差異性影響.方法 40只雄性SD大鼠,隨機(jī)分為5組,每組8只,分別為:單純SMD組、阿托品(Atropine)+SMD組、加拉明(Gallamine)+SMD組、4-二苯乙酰氧基-N-甲基哌淀甲碘化物(4-DAMP)+SMD組及加拉明+4-DAMP+SMD組.制備胃竇縱、環(huán)行平滑肌條,分別采用累積量加入四磨湯,以及阿托品(10^-6 mol/L)、加拉明(10^-6 mol/L)、4-DAMP(0.4×10^-6 mol/L)、加拉明+4-DAMP孵育肌條后再累積加入四磨湯,記錄平滑肌等長(zhǎng)收縮活動(dòng),比較縱、環(huán)行肌收縮的差異性.結(jié)果 四磨湯誘發(fā)胃竇縱、環(huán)行肌收縮活動(dòng)增加,但兩者間無(wú)明顯差異(P>0.05),非選擇性M受體阻斷劑阿托品及選擇性M₂受體阻斷劑加拉明對(duì)四磨湯誘發(fā)胃竇環(huán)行肌收縮活動(dòng)的抑制明顯強(qiáng)于縱行肌(P<0.05),選擇性M₃受體阻斷劑4-DAMP及加拉明+4-DAMP對(duì)四磨湯誘發(fā)胃竇縱行肌收縮活動(dòng)的抑制作用明顯強(qiáng)于環(huán)行肌(P<0.05).結(jié)論 M受體及其亞型參與了四磨湯對(duì)胃竇運(yùn)動(dòng)的調(diào)節(jié),胃竇環(huán)行肌收縮更多的依賴(lài)于M受體及其M₂亞型的激活,胃竇縱行肌收縮更多的依賴(lài)于M₃受體亞型的激活.

Objective To investigate the different effects of muscarinic (M) receptor and its subtype on Simo Decoction (SMD)- induced contraction of antral longitudinal and circular strips. Methods Forty SD rats were randomly divided into five groups including SMD, Atropine + SMD, Gallamine + SMD, 4-DAMP + SMD, and Gallamine + 4-DAMP + SMD groups, the spontaneous contraction of antral longitudinal and circular strips induced by SMD were noted in the organ bath in the presence or absence of atropine, Gallamine, 4-DAMP or Gallamine + 4-DAMP. Results SMD induced the contraction of longitudinal and circular strips increasing with no significant difference (P > 0.05), SMD-induced contractile activity of longitudinal strips after incubation with non-selective M receptor blocker atropine or selective M₂ receptor blocker gallamine was stronger than circular strips (P < 0.05), and after incubation with selective M₃ receptor blocker 4-DAMP or gallamine + 4-DAMP, SMD-induced contractility of cicular strips was stronger than longitudinal strips (P < 0.05). Conclusion SMD-induced contraction of antral circular strips is more dependent on activation of M and M₂ receptor than longitudinal strips. However, SMD-induced contraction of antral longitudinal strips is more dependent on activation of M₃ receptor than circular strips.

國(guó)家973計(jì)劃項(xiàng)目(2009CB523002)