目的 探討竹節(jié)香附素A對人肝細(xì)胞癌裸鼠移植瘤的抑制作用.方法 建立人肝癌裸鼠移植瘤模型,隨機(jī)將成瘤裸鼠分為3組,即竹節(jié)香附素A治療組、順鉑組及生理鹽水對照組,于種植瘤細(xì)胞后第3天開始ip藥物,治療過程中隔天記錄瘤體大小、各組抑瘤率及藥物對裸鼠生存率的影響及瘤組織病理學(xué)改變.結(jié)果 竹節(jié)香附素A治療組能明顯抑制人肝癌細(xì)胞裸鼠移植瘤生長,治療8 d竹節(jié)香附素A組及順鉑組與對照組相比已有明顯差異(F=5.579,P=0.015),抑瘤率分別達(dá)到48.97%和53.71%,隨著治療時間的延長差異更加顯著;治療結(jié)束后竹節(jié)香附素A組和順鉑組平均瘤體質(zhì)量較輕,與對照組相比差異具有統(tǒng)計學(xué)意義(F=17.96,P=0.000);與化療藥順鉑組相比瘤體大小及抑瘤率均未見差異.竹節(jié)香附素A對裸鼠體質(zhì)量影響較小,治療結(jié)束后竹節(jié)香附素A治療組裸鼠存活率為100%,順鉑治療組存活率僅為62.5%,兩組存活率相比差異有顯著性(P<0.05).病理學(xué)觀察竹節(jié)香附素A治療組瘤體小,腫瘤細(xì)胞稀疏減少.結(jié)論 竹節(jié)香附素A對人肝癌裸鼠移植瘤生長具有明顯抑制作用,且竹節(jié)香附素A毒性低,安全性好.

Objective To study the inhibiting effect of raddeanin A on xenograft tumor in nude mice with transplanted human hepatocellular carcinoma. Methods The xenograft tumor modes of human HCC were established in nude mice. These nude mice were randomly divided into three groups (raddeanin A, cisplatin, and normal saline). In all groups, intraperitoneal injection (1 time per day) of drugs will be given from the third day after transplanted human HCC. During the treatment, all groups' date of tumor size and tumor inhibition rate were recorded, every other day. To anlayze drugs' impact on the survival rate and the histopathological changes of xenograft tumor. Results (1) This study finds that raddeanin A has significant inhibitive effect on xenograft tumor in nude mice. There was statistical significant difference among raddeanin A treatment group, cisplatin treatment group and normal saline control group on the 8th day (F = 5.579, P = 0.015). In the former two treatment groups, the tumor inhibition rate were 48.97% and 53.71% at that day. With the treatment prolonged, the differences was more significant. The average weight of tumor is lighter in raddeanin A group and Cisplatin group, Which have statistically significant compared with the normal saline control group after treatment (F=17.96,P=0.000). Whereas the difference in tumor size and tumor inhibition rate between raddeanin A treatment group and cisplatin treatment group had not statistical significance(P > 0.05). (2) Raddeanin A had small bad effect on the nude mice physical: after treatment, survival rate of nude mice was 100% in raddeanin A treatment group and 62.5% in cisplatin treatment group, a significant difference between the two groups (P < 0.05). (3) Pathological observation of raddeanin A treatment group showed that raddeanin A could shrink the tumors and make tumor cells were arranged loosely. Conclusion These finds reveal that raddeanin A, with the advantage of mild toxicity and high safety, has significant inhibitive effect on xenograft tumor in nude mice with transplanted human hepatocellular carcinoma.

福建省自然科學(xué)基金項目(NO2012J01399)