目的 探討不同炮制溫度對人參酸性多糖抗氧化活性的影響.方法 ip四氧嘧啶200 mg/kg建立糖尿病小鼠模型,按血糖值隨機分為5組,即模型組、陽性組、生曬參酸性多糖組、100 ℃紅參酸性多糖組、120 ℃紅參酸性多糖組,每組12只,同時取健康小鼠做對照組,分別ig 100 mg/kg的人參酸性多糖,每天定時給藥1次,給藥體積為10 mL/kg體質(zhì)量,陽性組ig 100 mg/kg格列美脲,模型組和對照組ig等體積的0.9%生理鹽水.28 d后分別測定小鼠血清和肝組織中超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽過氧化物酶(GSH-Px)、維生素C(VC)和維生素E(VE)的量.結(jié)果 3種人參酸性多糖均可提高SOD、GSH-Px活性,降低MDA水平,且作用效果120℃紅參酸性多糖>100℃紅參酸性多糖>生曬參酸性多糖,并且能提高肝組織和血清中VC和VE的量,且120℃紅參酸性多糖效果最明顯.結(jié)論 不同炮制溫度的人參酸性多糖均有抗氧化作用,在一定范圍內(nèi),抗氧化性隨炮制溫度的升高而增強.

Objective To investiate the changes in antioxidant activities of water-soluble ginseng acid polysaccharides from Panax ginseng induced by steaming at different temperatures on alloxan-induced diabetic mice. Methods Ginseng acid polysaccharides were obtained from white ginseng, red ginseng (steamed ginseng at 100 ℃) and steamed ginseng (steamed ginseng at 100 ℃) by water extraction and ion-exchange chromatography. Mice were injected intraperitoneally with a single dose of 200 mg/kg body weight, and the mice with blood glucose levels higher than 16.8 mmol/L were used as diabetic mice and were selected for the scceeding experiments. After 28 d administration with ginseng acid polysaccharides, the activities of SOD, GSH-Px, and the content of MDA were determined. Results All of the three acid polysaccharides could significantly increase higher activities of SOD, GSH-Px, VC and VE in serum and liver and significantly lower content of MDA as compared with diabetic model group. Conclusion After treatment with ginseng acid polysaccharides for 4 weeks, the antioxidant activities in alloxan-induced diabetic mice were improved, and the activities of ginseng acid polysaccharides increased with steam treatment.

吉林省科技廳課題(20130206059YY)