哺乳動物卵巢有大量的腔前卵泡,其中大部分卵泡不能進行排卵,而卵泡發(fā)育和閉鎖的調節(jié)是一個高度復雜的過程.隨著對卵泡生長過程的深入研究,國內外已經建立了多種體外培養(yǎng)方法,而腔前卵泡體外培養(yǎng)已成為現今的研究熱點,并將其應用于雌性生殖毒理評價研究當中,為新藥評價研究提供了可靠的實驗工具.對哺乳動物腔前卵泡的體外培養(yǎng)中的胚胎來源、分離方法、培養(yǎng)條件和添加成分等幾個方面進行了闡述,為進一步完善腔前卵泡培養(yǎng)體系提供實驗依據.

Preantral follicles are of great abundance in mammalian ovaries and the vast majority (> 99.9%) never become ovulatory; Regulation of follicular development and atresia is a complicated process. With further research about the process of follicle growth, many in vitro models have been established at home and abroad, while in vitro culture of preantral follicles that is applied to evaluating female reproductive toxicology and provides a reliable experimental tool for new drug evaluation has become a hot spot in the current study. This paper reviews the various factors in vitro culture of preantral follicles, such as embryos, separation methods, cultural conditions, and additives, which would further achieve the system to provide experimental basis for the culture.