哺乳動(dòng)物卵巢有大量的腔前卵泡,其中大部分卵泡不能進(jìn)行排卵,而卵泡發(fā)育和閉鎖的調(diào)節(jié)是一個(gè)高度復(fù)雜的過(guò)程.隨著對(duì)卵泡生長(zhǎng)過(guò)程的深入研究,國(guó)內(nèi)外已經(jīng)建立了多種體外培養(yǎng)方法,而腔前卵泡體外培養(yǎng)已成為現(xiàn)今的研究熱點(diǎn),并將其應(yīng)用于雌性生殖毒理評(píng)價(jià)研究當(dāng)中,為新藥評(píng)價(jià)研究提供了可靠的實(shí)驗(yàn)工具.對(duì)哺乳動(dòng)物腔前卵泡的體外培養(yǎng)中的胚胎來(lái)源、分離方法、培養(yǎng)條件和添加成分等幾個(gè)方面進(jìn)行了闡述,為進(jìn)一步完善腔前卵泡培養(yǎng)體系提供實(shí)驗(yàn)依據(jù).

Preantral follicles are of great abundance in mammalian ovaries and the vast majority (> 99.9%) never become ovulatory; Regulation of follicular development and atresia is a complicated process. With further research about the process of follicle growth, many in vitro models have been established at home and abroad, while in vitro culture of preantral follicles that is applied to evaluating female reproductive toxicology and provides a reliable experimental tool for new drug evaluation has become a hot spot in the current study. This paper reviews the various factors in vitro culture of preantral follicles, such as embryos, separation methods, cultural conditions, and additives, which would further achieve the system to provide experimental basis for the culture.