目的 建立腎陽虛骨質(zhì)疏松大鼠病證結(jié)合動物模型。方法 大鼠按體質(zhì)量隨機分為3組, 分別為實驗組16只、去卵巢組16只及假手術(shù)組16只。實驗組大鼠采用雙側(cè)去卵巢并im氫化可的松的方法進行造模。造模期間對大鼠一般情況進行觀察及對生理狀態(tài)量化檢測。3個月后, 采用ELISA法檢測大鼠血清環(huán)磷酸腺苷(cAMP)、環(huán)磷酸鳥苷(cGMP)、雌二醇(E₂)、睪酮(T)、促腎上腺皮質(zhì)激素(ACTH)、三碘甲狀腺原氨酸(T₃)、四碘甲狀腺原氨酸(T₄)水平。用電子天平稱腎上腺, 甲狀腺及胸腺濕質(zhì)量, 并取股骨進行骨密度和骨礦含量的檢測。結(jié)果 給藥造模2周后, 實驗組大鼠出現(xiàn)毛發(fā)干枯、畏寒喜暖、卷縮弓背、小便清長等癥狀, 體質(zhì)量增長明顯遲緩, 血清cAMP水平較假手術(shù)組顯著下降。3個月后, 實驗組大鼠自發(fā)活動次數(shù)明顯減少, 血清cAMP、E₂、ACTH、T₃、T₄水平明顯下降, 骨密度、骨礦含量顯著降低, 腎上腺及甲狀腺系數(shù)明顯低于假手術(shù)組。結(jié)論 去勢并im氫化可的松建立腎陽虛骨質(zhì)疏松大鼠動物模型是一種可行的方法。

Objective To establish an animal model of osteoporosis with kidney yang deficiency syndrome in rats. Methods Rats were randomly divided into three groups by body weight, They were experimental, ovariectomized, and Sham operation groups (16 in each group). In the experimental group, rats were subjected to bilateral ovarian resection followed by im injection of hydrocortisone. The general conditions and physiological conditions were observed and measured during the period of the experiment. After three months, the serum levels of cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP), estrogen (E₂), testosterone (T), adrenocorticotropic hormone (ACTH), three typical thyroid original acid (T₃), and four typical thyroid original acid (T₄) were determined by ELISA method. The fresh weights of adrenal, thymus, and thyroid were collected by using electronic balance. In addition, the bone mineral density (BMD) and bone mineral content (BMC) were detected. Results Two weeks after hydrocortisone treatment, it was observed that the rats in the experimental group displayed kidney yang deficiency syndrome such as dry hairs, chills, back arched, and clear abundant urine. Correspondingly, the body weight increased slowly. The level of cAMP was significantly lower than that of rats in the Sham operation group. Three months later, spontaneous activities of rats in the experimental group markedly decreased, the content of cAMP, E₂, ACTH, T₃, and T₄ significantly decreased when compared with the rats in the Sham operation group. The BMD and BMC decreased obviously, showing a notable statistic difference between the experimental group and Sham operation group. The coefficient of adrenal and thyroid was lower than that of rats in the Sham operation group. Conclusion Bilateral ovarian resection followed by im injection of hydrocortisone may be a credible method for establishing the animal model of osteoporosis with kidney yang deficiency syndrome.

國家科技部863計劃:抗骨質(zhì)疏松海洋中藥制劑—資骨膠囊的研究(2013AA093003)