目的 對杜仲抗骨質(zhì)疏松的藥效成分進行初步篩選。方法 采用小鼠成骨細胞MC3T3-E1體外培養(yǎng)模型, 通過MTT法測定細胞增殖, ELISA方法測定堿性磷酸酶(ALP)活性, 觀察杜仲中槲皮素、京尼平苷及桃葉珊瑚苷對成骨細胞增殖和分化的影響。結(jié)果 槲皮素促MC3T3-E1細胞增殖的有效濃度為10^-6 mmol/L, 桃葉珊瑚苷則為10^-5 mmol/L;10^-4 μmol/L京尼平苷在干預(yù)后4 d, 才逐漸顯現(xiàn)出促進MC3T3-E1增殖作用。槲皮素(10^-5、10^-3 mmol/L)、京尼平苷(10^-3 mmol/L)和桃葉珊瑚苷(10^-3 mmol/L)可增加MC3T3-E1 ALP活性。結(jié)論 槲皮素、京尼平苷和桃葉珊瑚苷可促進成骨細胞的增殖和分化, 且作用強度具有濃度相關(guān)性和時間相關(guān)性, 可能為杜仲抗骨質(zhì)疏松的藥效物質(zhì)基礎(chǔ)。

Objective To screen the pharmaceutical ingredients from Eucommia ulmoides with the function on anti-osteoporosis. Methods The proliferation of osteoblast MC3T3-E1 in mice cultured in vitro was assayed using MTT and ALP activity was assayed using ELISA kit, in order to observe the effect of quercetin, geniposide, and aucubin from E. ulmoides on the proliferation and differentiation of osteoblast. Results The effective concentration of quercetin on MC3T3-E1 proliferation was 10^-6 mmol/L and aucubin was 10^-5 mmol/L. While geniposide at 10^-4 mmol/L could gradually increase the proliferation of MC3T3-E1. The ALP activity of MC3T3-E1 could be increased by quercetin (10^-5 and 10^-3 mmol/L), geniposide (10^-3 mmol/L), and aucubin (10^-3 mmol/L). Conclusion Quercetin, geniposide, and aucubin could promote the proliferation and differentiation of osteoblasts, which are the basic materials from E. ulmoides with the function of anti-osteoporosis and is in a concentration-and time-dependent manner.

“十二·五”國家科技支撐計劃課題(SQ2010BAJY1411-08)