目的 建立小腸灌流液中頭孢克洛的高效液相色譜(HPLC)分析方法, 考察蒲地藍(lán)消炎口服液對(duì)頭孢克洛在大鼠小腸吸收的影響, 為臨床聯(lián)合用藥的合理性提供實(shí)驗(yàn)依據(jù)。方法 采用大鼠在體單向腸灌流實(shí)驗(yàn)?zāi)Ｐ? 應(yīng)用重量法校正灌流液體積, HPLC測(cè)定灌流液中頭孢克洛, 計(jì)算頭孢克洛腸吸收參數(shù)。結(jié)果 頭孢克洛在各腸段均有良好吸收, 小腸不同部位頭孢克洛的吸收速率常數(shù)(K_a)與有效滲透系數(shù)(P_eff)大小為十二指腸> 空腸≈回腸, 各腸段間的吸收無(wú)統(tǒng)計(jì)學(xué)差異;聯(lián)合應(yīng)用蒲地藍(lán)消炎口服液時(shí), 頭孢克洛在全場(chǎng)段的吸收均有所減少, 十二指腸處顯著減少(P<0.05), 其他腸段處無(wú)統(tǒng)計(jì)學(xué)差異(P> 0.05)。結(jié)論 研究結(jié)果表明蒲地藍(lán)消炎口服液會(huì)影響頭孢克洛的小腸吸收, 因此兩藥聯(lián)合使用時(shí)產(chǎn)生較好的藥效不是通過(guò)促進(jìn)頭孢克洛的吸收而實(shí)現(xiàn)的, 而是二者共同作用的結(jié)果。

Objective To establish a method for the determination of cefaclor by HPLC and to investigate the effects of Pudilan on intestinal absorption of cefaclor in rats. Methods The rat single-pass intestinal perfusion technique was applied to investigatting the intestinal absorption of cefaclor, and the gravimetry was used to correct the perfusion volume. After the concentration of cefaclor in perfusate was determined by HPLC, the absorption parameters of cefaclor were calculated. Results Cefaclor was absorbed well in all parts of the small intestine. The absorption rate constants (K_a) and the effective permeability coefficients (P_eff) of cefaclor were ordered as follows: duodenum > jejunum ≈ ileum. There was no significant indifference in each segment (P > 0.05). When cefaclor was used combining with Pudilan together, the values of K_a and P_eff decreased in all segments and especially in duodenum (P < 0.05) comparing with using cefaclor only. Besides no significant difference in the parameters was found in jejunum and ileum (P > 0.05). Conclusion The above findings reveal that the intestinal absorption of cefaclor could be affected by Pudilan. Therefore the combined application of the two drugs has better efficacy which is not accomplished by promoting the absorption of cefaclor, while it may be the result of the synergic action of the two drugs.