目的 分析消巖湯干預(yù)下sh-survivin對人肺腺癌A549細(xì)胞增殖及凋亡的影響。方法 選擇人肺腺癌A549細(xì)胞, 采用中藥復(fù)方進(jìn)行干預(yù)及shRNA進(jìn)行轉(zhuǎn)染, 將轉(zhuǎn)染后的細(xì)胞分組, 通過MTT法觀察消巖湯對A549細(xì)胞生長的影響, 免疫組化法檢測survivin蛋白的表達(dá), 流式細(xì)胞儀檢測細(xì)胞凋亡率。結(jié)果 消巖湯能有效地抑制人肺腺癌A549的細(xì)胞增殖, 運(yùn)用消巖湯作用細(xì)胞24 h后, 侵襲的細(xì)胞數(shù)明顯減少, 該方作用48 h后可以誘導(dǎo)腫瘤細(xì)胞凋亡;消巖湯中劑量組與轉(zhuǎn)染sh-survivin組抑制率相當(dāng), 不同劑量的消巖湯均可協(xié)同sh-survivin使肺癌細(xì)胞凋亡率增加。結(jié)論 消巖湯可以通過干預(yù)腫瘤細(xì)胞凋亡抑制蛋白的表達(dá)而有效抑制腫瘤的發(fā)展。

Objective To analyze the effect of Xiaoyan Decoction intervened sh-survivin human lung adenocarcinoma A549 cell proliferation and apoptosis. Methods Selecting the human lung adenocarcinoma A549 cells, using the intervence of compound Chinese materia medica and shRNA transfection, after transfection cells wre grouped, the effect of Xiaoyan Decoction on the proliferation of human lung adenocarcinoma A549 cell was determined by MTT method, the Survivin protein expression was detected by immunohistochemical method, and the cell apoptosis rate was detected by flow cytometry instrument. Results Xiaoyan Decoction could effectively inhibit the cell proliferation of human lung adenocarcinoma A549; Using Xiaoyan Decoction on cells after 24 h, the number of cells was significantly decreased, the tumor cell apoptosis was induced after 48 h of the administration of Xiaoyan Decoction. The inhibitory rate of Xiaoyan Decoction in treatment dose group and transfection group was equal, the Xiaoyan Decoction at different dosage can be coordinated with sh-survivin to increase rate of lung cancer cell apoptosis. Conclusion Xiaoyan Decoction can inhibit the tumor development by intervening tumor cell apoptosis and depressing the protein expression..