目的 探索利用陽(yáng)離子化牛血清白蛋白(C-BSA)復(fù)制膜性腎病模型的可行性,探討模型特點(diǎn),以及模型對(duì)各項(xiàng)指標(biāo)的影響及規(guī)律.方法 模型組隔日尾iv給予C-BSA溶液5 mg/只,持續(xù)6周;造模停止后繼續(xù)觀察4周.造模期第4、6周以及觀察期第8、10周采集血樣、尿樣,測(cè)定血清生化指標(biāo)、血常規(guī)指標(biāo)、尿蛋白指標(biāo)等,并留取相關(guān)臟器,計(jì)算臟器系數(shù),并進(jìn)行病理學(xué)檢查.結(jié)果 造模期時(shí),血清總蛋白、白蛋白明顯下降,血脂明顯升高,肌酐、尿素氮變化不明顯;血漿黏度明顯升高,全血黏度變化不大;尿蛋白明顯增加,尿量減少,尿白細(xì)胞增加;血紅細(xì)胞、血紅蛋白降低;腎系數(shù)、脾系數(shù)明顯提高,體質(zhì)量明顯下降;模型組動(dòng)物腎臟可見(jiàn)不同程度的腎小管蛋白管型、腎小管擴(kuò)張、再生腎小管、間質(zhì)炎細(xì)胞浸潤(rùn)、腎小球鮑曼氏囊增厚.造模結(jié)束進(jìn)入觀察期后,模型出現(xiàn)一定程度的恢復(fù),各項(xiàng)指標(biāo)均向正常值趨近.結(jié)論 成功復(fù)制了大鼠C-BSA模型,模型穩(wěn)定,臨床表現(xiàn)典型,可重復(fù)性高.

Objective To explore the feasibility of using cationic bovine serum albumin (C-BSA) on producing membranous nephropathy rat model, and to discuss the characteristics and parameters of the model. Methods The SD rats model was established by iv injection with C-BSA (5 mg) every other day for 6 weeks. Then they were observed for 4 weeks after model establishment. At the end of 4, 6, 8, and 10 weeks the blood and urine samples were collected, the serum biochemical indexes, blood routine, urine protein, etc. were determined, the relevant organs were taken, viscera coefficients were calculated, and pathological examination was performed. Results During the model establishment, 1) the total proteins and albumin in serum were significantly decreased, serum lipids were increased obviously, and serum creatinine and urea nitrogen variation were not obvious changed; 2) the plasma viscosity was increased significantly, while whole blood viscosity was less changed; 3) the proteins were increased significantly, and the volume of urine was reduced, while leucocyte was increased in the urine; 4) the red blood cells and hemoglobin were decreased; 5) the coefficients of kidney and spleen were obviously increased, when the body weight was decreased obviously; 6) it was seen that varying degrees of renal tubular pipe type of protein, renal tubular expansion, regeneration of renal tubule and interstitial inflammatory cell infiltration, and thickening of glomerular bowman's capsule in the kidneys. In observation period in a certain degree of recovery, many indicators were approaching to the normal levels. Conclusion The C-BSA induces an optimal outcome, with stable models, typical clinical manifestation, and high repeatability.