目的 研究提取鮑姆纖孔菌中總?cè)频淖罴压に嚄l件,比較鮑姆纖孔菌子實(shí)體總?cè)坪途z體總?cè)频捏w外抗乳腺癌細(xì)胞(MCF-7)活性.方法 在單因素的基礎(chǔ)上運(yùn)用Box-Behnken中心組合設(shè)計(jì),以超聲溫度、超聲時(shí)間、乙醇濃度3個(gè)因素3水平的實(shí)驗(yàn)?zāi)Ｐ?建立提取回歸方程.以響應(yīng)曲面法(RSM)對(duì)實(shí)驗(yàn)結(jié)果進(jìn)行分析,對(duì)鮑姆纖孔菌總?cè)铺崛」に囘M(jìn)行優(yōu)化;用CASY細(xì)胞分析儀檢測(cè)細(xì)胞活力,TUNEL法檢測(cè)細(xì)胞凋亡,分析鮑姆纖孔菌子實(shí)體總?cè)坪途z體總?cè)埔种迫橄侔┘?xì)胞增殖和促進(jìn)乳腺癌細(xì)胞凋亡活性.結(jié)果 修正最優(yōu)條件為超聲時(shí)間40 min,乙醇體積分?jǐn)?shù)為70%,超聲溫度為60 ℃,此條件下總?cè)频淖罡咛崛÷蕿?.83 mg/g.驗(yàn)證實(shí)驗(yàn)結(jié)果平均提取率為9.81 mg/g,與模型預(yù)測(cè)值較為接近;鮑姆纖孔菌子實(shí)體總?cè)圃?.2 mg/mL時(shí),菌絲體總?cè)圃?.4 mg/mL時(shí),對(duì)MCF-7細(xì)胞的增殖有顯著的抑制,抑制效果呈濃度相關(guān)性.鮑姆纖孔菌子實(shí)體總?cè)圃?.2 mg/mL時(shí),菌絲體總?cè)圃?.4 mg/mL時(shí),能顯著增強(qiáng)MCF-7細(xì)胞的凋亡,增強(qiáng)效果呈濃度相關(guān)性.結(jié)論 曲面響應(yīng)法可以對(duì)鮑姆纖孔菌總?cè)频奶崛」に囘M(jìn)行優(yōu)化;鮑姆纖孔菌三萜對(duì)乳腺癌細(xì)胞(MCF-7)有明顯的抑增殖活性和凋亡誘導(dǎo)作用,呈濃度相關(guān)性,且子實(shí)體總?cè)票染z體總?cè)企w外抗乳腺癌細(xì)胞(MCF-7)活性更強(qiáng).

Objective To optimize the ultrasonic-assisted extraction technology of total triterpenoids from Inonotus baumii, and compare the inhibitory activities of total triterpenoids from fruiting body and from mycelium on breast cancer cells (MCF-7) in vitro. Methods A three-factor and three-level Box-Behnken experimental design was employed using ultrasonic temperature, ultrasonic time and ethanol concentration as a model. Response surface method (RSM) was used to analyze the result and optimize the extracting process. The anticancer activities of total triterpenoids from fruiting body and from mycelium against MCF-7 were completed by CASY cell analyzer and TUNEL method, respectively. Results Optimum extraction conditions were determined as follows: extraction time of 40 min, concentration of ethanol 70%, and extraction temperature of 60 ℃. Under these conditions, the yield of total triterpenoids reached 9.83 mg/g. The experimental results were verified with an average yield of 9.81 mg/g, which is close to the model predicted value. The concentration of total triterpenoids from fruiting body of I. baumii reached 0.2 mg/mL, and that from mycelium reached 0.4 mg/mL, which could inhibit hyperplasia of MCF-7 in dose-dependent manner. The concentration of total triterpenoids from fruiting body of I. baumii reached 0.2 mg/mL, and that from mycelium reached 0.4 mg/mL, which could significantly enhanced MCF-7 apoptosis in dose-dependent manner. Conclusion The RSM technology optimization for extracting total triterpenoids from I. baumii is suitable. Total triterpenoids from fruiting body and mycelium of I. baumii could inhibit proliferation of MCF-7, and induced apoptosis on tumor cells in dose-dependent manner. Total triterpenoids from fruiting body are more active than that from mycelium against MCF-7.