目的 比較降壓避風(fēng)片新老配方的降壓效果及作用特點(diǎn).方法 選用原發(fā)性高血壓大鼠(SHR)及其對(duì)照性WKY大鼠進(jìn)行對(duì)比試驗(yàn).實(shí)驗(yàn)動(dòng)物分為8組:WKY大鼠組、SHR對(duì)照組、降壓避風(fēng)片老配方組高、中、低劑量(1.00、0.50、0.25 g藥粉/kg)組、降壓避風(fēng)片新配方高、中、低劑量(1.00、0.50、0.25 g藥粉/kg)組.降壓試驗(yàn)采用大鼠無(wú)創(chuàng)血壓測(cè)定儀對(duì)大鼠尾動(dòng)脈進(jìn)行血壓測(cè)定,分為急性降壓作用試驗(yàn)和慢性降壓驗(yàn).急性降壓測(cè)定給藥前、給藥后1、3 h血壓值;慢性降壓測(cè)定連續(xù)給藥后6、11、14 d血壓值.連續(xù)給藥14 d后,進(jìn)行大鼠心臟超聲心動(dòng)試驗(yàn),監(jiān)測(cè)大鼠室間隔厚度、左室內(nèi)徑、左室后壁、射血分?jǐn)?shù)、左室短軸縮短率、左心室心肌質(zhì)量、左心室容量等;后腹主動(dòng)脈取血,分別測(cè)定血清NO、總蛋白、白蛋白、球蛋白、丙氨酸氨基轉(zhuǎn)移酶、天門(mén)冬氨酸氨基轉(zhuǎn)移酶、尿素、肌酐、葡萄糖、三酰甘油、低密度脂蛋白和血漿內(nèi)皮素、腎素活性及血管緊張素Ⅱ含量.結(jié)果 急性降壓試驗(yàn):各劑量組均可顯著降低藥后3 h的收縮壓(P <0.01),新老配方相應(yīng)劑量間無(wú)顯著差異;慢性降壓試驗(yàn):SHR組心率顯著快于WKY組,用藥后,各劑量組均無(wú)顯著降低心率的效果.結(jié)論 新老配方均可平穩(wěn)降低SHR血壓,降壓作用確切.

Objective To compare the antihypertensive effect and function characteristic of the old and new Jiangya Bifeng formula. Methods spontaneously hypertensive rats (SHR) and its controlled WKY rats were employed in the comparative study. Experimental animals were divided into 8 groups: WKY rats group, SHR control group, old Jiangya Bifeng formula of high, medium and low dose group, new Jiangya Bifeng formula of high, medium and low dose group. Step-down test adopted the rat noninvasive blood pressure meter on rat tail artery blood pressure measurement, divided into acute antihypertensive effect test and chronic step-down test. In the acute antihypertensive study, blood pressure value were tested before the drug delivery and after 1, 3 h; In the chronic antihypertensive study, the blood pressure values were determined continuous dosing after 6, 11, 14 d. After 14 days continuous dosing,the level of Echocardiographic heart、IVS、LVID、LVPW、EF、FS、LV Mass、LV Vol were determined.After posterior abdominal aortic blood, blood were tested: Serum NO, total protein, albumin, globulin, alanine aminotransferase, aspartate aminotransferase, urea, creatinine, glucose, triglycerides, low-density lipoprotein and plasma endothelin, renin activity and angiotensin Ⅱ content. Results acute anti-hypertension test: 3 h after Jiangya Bifeng formula administration the blood pressure were significantly reduced. However, there was no difference between the old and new formula (P <0.01); Chronic anti-hypertension test: The heart rate of SHR was significantly faster than WKY rats, after drugs administration, the heart rate were not significantly affected by both old and new Jiangya Bifeng formula. Conclusion the new and old Jiangya Bifeng formula could smoothly reduce the blood pressure, and both have a reliable antihypertensive effect.