目的 研究人參皂苷提取物(GE)對(duì)酒精性肝損傷的保護(hù)作用與作用機(jī)制.方法 將ICR雄性小鼠分為正常對(duì)照組、陽性對(duì)照組(聯(lián)苯雙酯)、模型組及人參皂苷提取物高、中、低劑量組,建立慢性酒精性肝損傷模型,用人參皂苷提取物進(jìn)行干預(yù),ig給藥30 d后,稱取肝質(zhì)量,計(jì)算肝臟系數(shù),測定小鼠血清丙氨酸氨基轉(zhuǎn)移酶(ALT)和天門冬氨酸氨基轉(zhuǎn)移酶(AST)活力、三酰甘油(TG)、肝臟內(nèi)丙二醛(MDA)和還原型谷胱甘肽(GSH)的含量,HE染色觀察小鼠肝臟病理變化,綜合評(píng)價(jià)人參皂苷提取物對(duì)小鼠酒精性肝損傷的保護(hù)作用.結(jié)果 人參皂苷提取物各劑量組能明顯降低酒精性肝損傷小鼠的肝臟系數(shù)(P <0.05)、血清TG的含量(P <0.01),可降低其血清中ALT、AST活力(P <0.05或P <0.01),但與正常組ALT、AST活力仍有差異(P <0.05);可在一定程度上降低肝組織中MDA含量(P <0.01),增高肝組織中GSH含量(P <0.01),減少肝組織病理損傷.結(jié)論 人參皂苷提取物對(duì)酒精性肝損傷有一定保護(hù)作用,其機(jī)制可能與抑制肝內(nèi)脂肪堆積,抗氧化作用有關(guān).

Objective To investigate the hepatoprotective effects of ginsenosides extract (GE) on alcoholic-induced liver injury in mice. Methods ICR mice were randomly divided into six groups, including negative control group, positive control group (Bifendate), model group, and low-, mid-, high-dose GE groups. The model of alcoholic-induced liver injury was induced in ICR mice by 56° alcohol. The liver injury mice were intervened by GE for 30 d followed by liver weight and liver index. Meanwhile, the contents of serum alanine aminotransferase (ALT) and aspertate aminotransferase (AST) energy, triglyceride (TG), and malondialdehyde (MDA) and glutathione (GSH) in the liver were determined. The pathological changes in mice liver were also observed. Comprehensive evaluation of GE on the protective effects of alcoholic liver injury in mice was performed. Results GE could obviously reduce the liver weight coefficient of alcoholic-induced liver injury in mice (P <0.05) and the content of TG in serum (P <0.01). The levels of ALT and AST in serum could be reduced (P <0.05 or 0.01), but it had difference from the normal group (P <0.05). To a certain extent, GE can reduce the MDA content in liver tissue homogenate (P <0.01), increase the content of GSH in liver tissue homogenate (P <0.01) and reduce the liver tissue pathological damage. Conclusion GE has certain protective effect on alcoholic liver injury in mice. The inhibitory effect of fat accumulation in the liver is more apparent. Ginsenosides could protect liver through the mechanism of anti lipid peroxidation.

吉林省科技發(fā)展計(jì)劃(20130303101YY);公益性行業(yè)(農(nóng)業(yè))專項(xiàng)(2013031106)