目的 采用HPLC法測定紅參提取物中α-亞麻酸、亞油酸的量,為紅參的質(zhì)量控制提供參考依據(jù).方法 采用DIKMA Diamonsil C₁₈(250 mm×4.6 mm,5 μm)色譜柱,以乙腈-0.1%磷酸水(90:10)為流動相,體積流量1.0 mL/min,檢測波長203 nm,柱溫30 ℃.結果 在此條件下,α-亞麻酸、亞油酸與其他組分能得到良好的分離,α-亞麻酸、亞油酸的進樣質(zhì)量分別在0.066～0.33 μg(r＝0.999 9)、1.39～6.96 μg(r＝0.999 9)與各自峰面積積分值呈良好線性關系,專屬性、精密度、穩(wěn)定性、加樣回收率均符合中藥含量測定要求.結論 本方法可用于測定紅參中的α-亞麻酸、亞油酸,為紅參的質(zhì)量監(jiān)控提供簡便、快速的方法.

Objective To determine the contents of α-linolenic acid and linoleic acid in red ginseng extract by HPLC, and to provide reference for the quality control of red ginseng. Methods Using DIKMA Diamonsil C₁₈ (250 mm × 4.6 mm, 5 μm) column, acetonitrile 0.1% phosphoric acid water (90:10) as mobile phase, flow rate of 1.0 mL/min, the detection wavelength was 203 nm. The column temperature was 30 ℃. Results Under this condition, α-linolenic acid, linoleic acid and other components could get good separation, and the sample injection contents of α-linolenic acid and linoleic acid respectively were in the range of 0.066—0.33 μg (r = 0.999 9), 1.39—6.96 μg (r = 0.999 9) and their peak area values showed a good linear relationship. The specificity, precision, stability, and recovery rate all met the requirements of content determination of Chinese materia medica. Conclusion This method can be used for the determination of α-linolenic acid and linoleic acid contents in red ginseng, providing the method is simple and fast for the quality control of red ginseng.

科技重大專項"重大新藥創(chuàng)制"(2013ZX09402202)