目的 探討復(fù)方腸泰對葡聚糖硫酸鈉(DSS)誘導(dǎo)的小鼠慢性潰瘍性結(jié)腸炎(UC)的治療作用。方法 隨機(jī)將C57BL/6小鼠分為正常對照組、DSS模型組、復(fù)方腸泰給藥組。采用2% DSS溶液對小鼠進(jìn)行造模,小鼠連續(xù)5 d自由飲用DSS水,接著連續(xù)5 d自由飲水,重復(fù)3個(gè)循環(huán)。復(fù)方腸泰組每天按照18 g/kg ig給藥,其他組ig給予相同體積的蒸餾水。每天記錄小鼠體質(zhì)量、疾病活動(dòng)指數(shù)(DAI)評分結(jié)果,采用HE染色觀察結(jié)直腸組織病理學(xué)變化,給予組織病理學(xué)評分;ELISA檢測TNF-α、IL-10表達(dá)水平,免疫組化檢測COX-2蛋白表達(dá)情況。結(jié)果 與DSS模型組比較,復(fù)方腸泰組小鼠疾病狀態(tài)得到明顯改善,DAI和組織病理學(xué)評分顯著降低,炎癥因子IL-10表達(dá)升高,TNF-α、COX-2蛋白的表達(dá)降低。結(jié)論 復(fù)方腸泰對小鼠慢性UC具有顯著地治療作用,可能通過促進(jìn)抗炎癥因子IL-10的表達(dá),同時(shí)抑制促炎癥因子TNF-α、COX-2蛋白的表達(dá)發(fā)揮作用。

Objective To investigate the therapeutic effect of Fufang Changtai on dextran sulfate sodium (DSS)-induced chronic ulcerative colitis (UC) in mice. Methods C57BL/6 mice were randomly divided into three groups: Vehicle group, DSS model group, and Fufang Changtai group. The model of colitis in mice was induced by drinking water containing 2% DSS for 5 d, followed by 5 d of normal water for 3 times. In Fufang Changtai group, the mice were ig given Fufang Changtai 18 g/kg per day during DSS treatment. Mice in Vehicle group and DSS model group were given the same volume of water, instead. Body weight and disease activity index (DAI) score were recorded every day. The colorectal mucosal histological changes were observed by HE staining. Tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) levels in colorectal tissue were determined by ELISA. The expression of COX-2 was determined by immunohistochemistry. Results Compared with the DSS groups, Fufang Changtai group could significantly improve the symptoms, reduce colonic DAI and histological scores. The levels of TNF-α and COX-2 were markedly decreased accompanying with IL-10 content increasing by the treatment of Fufang Changtai. Conclusion Fufang Changtai has the therapeutic effects on DSS-induced UC in mice, which is probably mediated by the enhancement of anti-inflammatory cytokines IL-10 and the inhibition of pro-inflammatory cytokines TNF-α and COX-2.

國家自然科學(xué)基金(81302968);中國中醫(yī)科學(xué)院自主選題課題(ZZ08080012)