目的 研究太行菊Opisthopappus taihangensis和野菊花Dendranthema indicum不同部位、不同濃度水提取物對(duì)乙型肝炎病毒(HBV)的抑制作用。方法 提取并配置不同濃度的太行菊和野菊花的花和莖部位的水提取液,分別以拉米夫定(3TC)和綠茶提取物(GTE)作對(duì)照,采用酶聯(lián)免疫反應(yīng)檢測(cè)太行菊花和莖提取物對(duì)HBsAg、HBeAg的抑制作用,采用實(shí)時(shí)定量PCR檢測(cè)不同濃度提取物對(duì)HBV復(fù)制過(guò)程中HBV RNA的影響,熒光素酶報(bào)告基因檢測(cè)不同提取物對(duì)HBV轉(zhuǎn)錄的影響。結(jié)果 酶聯(lián)免疫反應(yīng)結(jié)果表明,1μg/mL太行菊莖提取物能夠在作用48、96 h時(shí)抑制HBsAg和HBeAg,而1μg/mL野菊花莖提取物只能在作用48 h時(shí)抑制HBsAg和HBeAg;0.1μg/mL太行菊花提取物與1μg/mL野菊花花提取物對(duì)HBsAg和HBeAg的抑制作用相當(dāng)。熒光素酶報(bào)告基因檢測(cè)結(jié)果說(shuō)明,太行菊花和莖提取物相對(duì)野菊花和莖提取物對(duì)HBV啟動(dòng)子質(zhì)粒CP有抑制作用。PCR結(jié)果顯示,太行菊花和莖提取物在低濃度時(shí)對(duì)HBV RNA合成具有一定的抑制作用。結(jié)論 太行菊水提取物和野菊花提取物都能夠不同程度抗HBV,但太行菊效果優(yōu)于野菊花。

Objective To investigate the inhibitory effect of different parts and different concentration extracts from Opisthopappus taihangensis and Dendranthema indicum on Hepatitis B virus. Methods The solutions of flowers and stems from O. taihangensis and D. indicum with different concentration were extracted and prepared, respectively. Lamivudine (3TC) and green tea extract (GTE) were used as controls. Inhibition of HBsAg and HBeAg were detected using enzyme-linked immune response. The effect of different concentrations of extracts on RNA HBV in HBV replication were detected by real time quantitative PCR. And effect of different extracts on HBV transcription were detected by Luciferase report gene. Results Both HBsAg and HBeAg expression were decreased significantly post-treatment 48 h, 96 h with 1 μg/mL O. taihangensis stem extracts. D. indicum stem extracts (1 μg/mL) could suppress HBsAg and HBeAg at 48 h. The inhibition of 0.1 μg/mL O. taihangensis flower extracts on HBV could reach the effect of 1 μg/mL D. indicum flower extracts. The luciferase reporter gene detection results showed the inhibition of O. taihangensis stem extracts and flower extracts on Luciferase HBV CP. PCR results showed that O. taihangensis stem extracts and flower extracts could inhibit the synthesis of HBV RNA at low concentration. Conclusion Both O. taihangensis and D. indicum extracts have anti-HBV effects. And this anti-viral effect of O. taihangensis is better than that of D. indicum.

國(guó)家自然科學(xué)基金資助項(xiàng)目(31201332)