目的 探討不同固定液和固定時(shí)間對(duì)大鼠睪丸組織病理學(xué)制片質(zhì)量的影響。方法 選用雄性SD大鼠12只,取兩側(cè)睪丸組織,共24例標(biāo)本。每例標(biāo)本分別用不同的固定液(10%中性福爾馬林溶液、30%Davidson固定液及FAA固定液)固定不同的時(shí)間(24、48 h)后,進(jìn)行脫水、包埋、切片、HE染色。用LEICA DM5000B普通光學(xué)顯微鏡觀察。結(jié)果 3種固定液固定24 h后,可見(jiàn)30% Davidsons固定液組睪丸組織的異常變化程度低于10%中性福爾馬林組和FAA組;固定時(shí)間對(duì)睪丸組織有一定程度的影響:固定24 h的異常變化程度低于固定48 h。結(jié)論 30% Davidson固定液組SD大鼠睪丸組織病理學(xué)變化程度最小;固定時(shí)間不宜超過(guò)24 h。

Objective To investigate the effects of different fixatives and fixation time on testis histopathology of rats. Methods A total of 24 testis tissue samples were collected from 12 male rats. Every specimen was for dehydration, embedding, sectioning, and HE staining after fixing for 24 or 48 h in different fixatives (10% neutral buffered formalin, 30% Davidsons fixative, and FAA fixative), then the tissues were observed by LEICA DM5000B ordinary optical microscopy. Results The testis histopathology changes by 30% Davidsons fixative were less than those by 10% neutral buffered formalin and FAA fixative after fixed for 24 h; The fixation time had a certain effect on testis tissue: histopathology change of 24 h was less than that of 48 h. Conclusion Abnormal change of testis histopathology in rats in 30% Davidsons fixative was minimized; Fixation time should not be more than 24 h.