目的 研究銀翹解毒丸各工藝因素對(duì)綠原酸轉(zhuǎn)移率的影響,為制備工藝優(yōu)化及質(zhì)量標(biāo)準(zhǔn)修訂提供實(shí)驗(yàn)數(shù)據(jù)。方法 按《中國藥典》所載銀翹解毒丸處方和制法,用HPLC跟蹤綠原酸在各工藝步驟中的轉(zhuǎn)移率;單因素考察提取溫度、濃縮溫度、干燥溫度對(duì)綠原酸轉(zhuǎn)移率的影響。結(jié)果 處方飲片粉碎后,經(jīng)提取、濃縮、干燥、制丸4個(gè)環(huán)節(jié)綠原酸的轉(zhuǎn)移率分別為78.0%、69.53%、55.90%、55.41%;制備過程中溫度是影響綠原酸轉(zhuǎn)移率的主要因素。結(jié)論 規(guī)范銀翹解毒丸的制備工藝條件可提高綠原酸的轉(zhuǎn)化率,保證產(chǎn)品質(zhì)量穩(wěn)定可控。

Objective To investigate the transfer rates of chlorgenic acid in the preparation process of Yinqiao Jiedu Pills (YJP), and to provide experimental data for technical improvement and quality control. Methods According to the recipe and technique of YJP contained in pharmacopoeia, the transfer rates of chlorgenic acid in the preparation process of YJP were determined by HPLC. Meanwhile, single factor experiment was used to investigate the effect of extraction temperature, condensation temperature, and drying temperature on the transfer ratio of chlorogenic acid. Results The preparation process contained four steps: extraction, concentration, drying, and pelletizing. The mean transfer rates of chlorgenic acid in above step were 78.0%, 69.53%, 55.90%, and 55.41%, and in the process of preparation, temperature was the main factor affecting the transfer ratio of chlorogenic acid. Conclusion Standardization of YJP preparation conditions can improve the conversion rate of chlorogenic acid, to ensure the quality stable and controllable.

江蘇省衛(wèi)生廳"銀翹解毒系列制劑中綠原酸轉(zhuǎn)移率的影響因素研究"項(xiàng)目(J201113);連云港市"521 高層次人才培養(yǎng)工程"項(xiàng)目