目的 建立癃閉舒片中補(bǔ)骨脂素和異補(bǔ)骨脂素的定量方法。方法 對(duì)國(guó)內(nèi)3個(gè)企業(yè)生產(chǎn)的6個(gè)批次的癃閉舒片,采用HPLC法檢測(cè),Agilent TC-C₁₈(2)色譜柱(250 mm×4.6 mm,5μm),流動(dòng)相是甲醇-水(43:57),體積流量1.0 mL/min,檢測(cè)波長(zhǎng)為246 nm,柱溫30℃。結(jié)果 補(bǔ)骨脂素回歸方程為:Y=84.44X+9.994,r=0.9999(n=12)。異補(bǔ)骨脂素回歸方程為:Y=82.434X+8.8952,r=0.9999(n=12)。補(bǔ)骨脂素和異補(bǔ)骨脂素的線性范圍均在4.04~151.5μg。結(jié)論 本方法操作簡(jiǎn)便、準(zhǔn)確、專屬性強(qiáng)、穩(wěn)定,可用于癃閉舒片中補(bǔ)骨脂素和異補(bǔ)骨脂素的含量測(cè)定。

Objective To establish a method for the content determination of psoralen and isopsoralen in Longbishu Pills (LBSP). Methods HPLC was performed on the Agilent TC-C₁₈(2) column (250 mm×4.6 mm, 5 μm) with the mobile phase of MeOH-H2O (43:57), the flow rate was 0.8 mL/min, the detection wavelength 246 nm, and the column temperature was 30℃. Results The element regression equation of psoralen was Y=84.44X+9.994, r=0.999 9 (n=12). The element regression equation of isopsoralen was Y=82.434X+8.895 2, r=0.999 9 (n=12). The concentrations of psoralen and isopsoralen were within the range of 4.04-151.5 μg. Conclusion The method is simple, reliable, and specific, with good reproducibility, and could be used to determine the content of psoralen and isopsoralen in Longbishu Pills.

遼寧省自然科學(xué)基金優(yōu)秀人才培養(yǎng)項(xiàng)目(2015020698)