[關(guān)鍵詞]
[摘要]
目的 觀察芒柄花素對(duì)不同亞型乳腺癌細(xì)胞周期相關(guān)基因和蛋白表達(dá)的影響。方法 乳腺癌細(xì)胞MCF-7、SK-BR-3、MDA-MB-231經(jīng)0、40、80 μmol/L芒柄花素作用48 h后,分別采用Trizol和RIPA裂解細(xì)胞,收集細(xì)胞的總RNA和總蛋白,進(jìn)一步采用實(shí)時(shí)定量PCR技術(shù)和Western blotting技術(shù)檢測(cè)細(xì)胞周期調(diào)控因子cyclin D1、cyclin E、p21、p27的基因和蛋白表達(dá)情況。結(jié)果 3種乳腺癌細(xì)胞cyclin D1和cyclin E的基因及蛋白表達(dá)水平隨著藥物濃度的增加而降低,而p21和p27的基因和蛋白表達(dá)水平隨藥物濃度的增加而升高,與對(duì)照組比較,差異顯著(P<0.05、0.01)。結(jié)論 芒柄花素可以通過調(diào)控cyclin D1、cyclin E、p21和p27基因和蛋白的表達(dá),將乳腺癌細(xì)胞阻滯于G1期,從而抑制乳腺癌細(xì)胞的增殖。
[Key word]
[Abstract]
Objective To investigate the effect of formononetin on expression of cell cycle related genes and proteins in different subtypes of breast cancer cell lines. Methods Three subtypes of breast cancer cell lines, MCF-7, SK-BR-3 and MDA-MB-231, were treated with different concentrations of formononetin for 48 h. Total RNA and protein from breast cancer cells were extracted with Trizol and RIPA method. Then the gene and protein expression of cyclin D1, cyclin E, p21 and p27 were assayed by real-time PCR and Western blotting, respectively. Results After treated with formononetin, the levels of gene and protein expression of cyclin D1 and cyclin E were down-regulated in breast cancer cells, however those of p21 and p27 were up-regulated. Conclusion Formononetin could arrest breast cancer cell in G1 phase, through regulating the gene and protein expression of cyclin D1, cyclin E, p21 and p27.
[中圖分類號(hào)]
[基金項(xiàng)目]
福建省自然科學(xué)基金青年創(chuàng)新項(xiàng)目(2014D013);廈門市科技計(jì)劃項(xiàng)目(3502Z20154052);廈門市中醫(yī)后備人才項(xiàng)目