目的 觀察紫花牡荊素(CAT)對人宮頸癌HeLa和SiHa細(xì)胞凋亡和侵襲轉(zhuǎn)移的影響。方法 不同濃度CAT作用于HeLa和SiHa細(xì)胞不同時(shí)間后,采用MTT法觀察藥物對細(xì)胞活力的影響,流式細(xì)胞儀檢測凋亡率,劃痕試驗(yàn)觀察細(xì)胞遷移率,基質(zhì)膠法測定細(xì)胞粘附百分率,Transwell試驗(yàn)檢測侵襲細(xì)胞數(shù),Western blotting分析CAT對SiHa細(xì)胞抑制轉(zhuǎn)移蛋白nm23-H1和促轉(zhuǎn)移蛋白MTA1表達(dá)的影響。結(jié)果 CAT顯著抑制HeLa和SiHa細(xì)胞活力,增加細(xì)胞凋亡率;降低細(xì)胞遷移百分率和黏附百分率;減少侵襲細(xì)胞數(shù);明顯增加SiHa細(xì)胞nm23-H1蛋白表達(dá),降低MTA1蛋白表達(dá)。結(jié)論 CAT顯著誘導(dǎo)人宮頸癌HeLa和SiHa細(xì)胞凋亡,抑制其侵襲遷移,提示其具有潛在的抗宮頸癌作用。

Objective To examine whether casticin influences the apoptosis, migration, and invasion of human cervical cancer HeLa and SiHa cells. Methods HeLa and SiHa cells were treated with various concentration of casticin, cell viability was examined by MTT assay and the apoptosis induced by casticin was determined by flow cytometry. The scratch test, matrigel assay, and Transwell test were performed respectively to estimate the capabilities of migration, adhesion, and invasion of HeLa and SiHa cells. The protein expression of nm23-H1 and MTA1 in SiHa cells was analyzed by Western blotting. Results Casticin treatment significantly inhibited cell viability, increased apoptosis rate, decreased migration percentages and adhesion of HeLa and SiHa cells, and reduced invasion cells number. The protein expression of nm23-H1 was evidently enhanced, but that of MTA1 was significantly decreased. Conclusion Casticin markedly induces the apoptosis and suppresses the migration and invasion of HeLa and SiHa cells, suggesting its potential therapeutic effects on cervical cancer.

上海市衛(wèi)計(jì)委科研項(xiàng)目資助(201440542)