目的 探討女貞子提取物對(duì)糖尿病小鼠的降血糖作用及抗氧化活性。方法 以丁羥甲苯(BHT)、維生素C為陽(yáng)性對(duì)照,鐵離子還原法、DPPH·自由基清除法測(cè)定女貞子提取物體外抗氧化活性;以阿卡波糖為陽(yáng)性對(duì)照,測(cè)定女貞子提取物抑制α-葡萄糖苷酶的活性。昆明種小鼠隨機(jī)分為對(duì)照組、模型組、消渴丸(陽(yáng)性藥,1.0 g/kg)組和女貞子提取物低、高劑量(200、800 mg/kg)組,每組8只,以鏈脲佐菌素(STZ)結(jié)合高脂飼料誘導(dǎo)糖尿病小鼠模型,給藥治療3周。每周應(yīng)用血糖儀檢測(cè)1次空腹血糖水平;進(jìn)行口服葡萄糖耐量試驗(yàn)(OGTT);試劑盒法檢測(cè)血清胰島素、糖化血紅蛋白(HbA1c)、總膽固醇(TC)、三酰甘油(TG)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)、谷胱甘肽過(guò)氧化物酶(GSH)、超氧化物歧化酶(SOD)、丙二醛(MDA)、尿素氮(BUN)水平。結(jié)果 女貞子提取物對(duì)鐵離子還原能力較BHT和Vc差,對(duì)DPPH自由基的清除能力較BHT強(qiáng);對(duì)α-葡萄糖苷酶的抑制作用強(qiáng)于阿卡波糖;與模型組比較,女貞子提取物組小鼠血糖水平顯著降低,血清TC、TG、LDL-C、HbA1c、MDA及BUN水平顯著降低,胰島素、HDL-C、SOD和GSH水平顯著升高,且存在明顯的劑量依賴性。結(jié)論 女貞子提取物具有顯著的降血糖及抗氧化活性。

Objective To investigate the protective effects of hypoglycemic and antioxidant activity of extracts from Ligustrum lucidum on experimental diabetic. Methods With dibutyl hydroxy toluene (BHT) and vitamin C as positive control, to determine the in vitro anti-oxidant activity of extracts from L. lucidum by iron reduction and DPPH free radical scavenging method; With acarbose as positive control, to determine the activity of extracts from L. lucidum to inhibit α-glucosidase. Kunming mice were randomly divided into five groups:control group, model group, Xiaoke Pill (positive drug, 1.0 g/kg) group, low- and high-dose (200 and 800 mg/kg) extracts from L. lucidum groups, with eight rats in each group. Use streptozocin (STZ) combined with high fat diet to establish diabetes model, then mice were treated with drug for 3 weeks. Detect the level of fasting blood glucose by the application of blood glucose meter, do oral glucose tolerance test (OGTT) and detect the levels of insulin, glycosylated hemoglobin (HbA1c), total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL), high density lipoprote (HDL-C), glutathione peroxidase (GSH), superoxide dismutase (SOD), malondialdehyde (MDA), and blood urea nitrogen (BUN) in serum using assay kit. Result The effect of extracts from L. lucidum on the iron reduction was higher than that of BHT and VC, the effect on DPPH· free radical scavenging was stronger than BHT, and the effect on alpha glucosidase inhibition was stronger than that of acarbose. Compared with model group, mice blood glucose levels of extracts from L. lucidum group decreased significantly, serum TC, TG, LDL-C, HbA1c, MDA, and BUN levels decreased significantly, the levels of insulin, high-density lipoprotein cholesterol (HDL-C), SOD, and GSH were significantly increased, and there was dose dependence. Conclusion Extracts from L. lucidum has significant hypoglycemic and anti-oxidant activity.

國(guó)家公益性行業(yè)科研專項(xiàng)(201303111);吉林省科技發(fā)展計(jì)劃項(xiàng)(20140204013YY,20150307012YY)