目的 觀察炎調(diào)方對膿毒癥急性肺損傷(ALI)大鼠肺組織髓過氧化物酶(MPO)、丙二醛(MDA)水平的影響。方法 清潔級健康雄性SD大鼠隨機分為對照組、假手術(shù)組、模型組、炎調(diào)方組、地塞米松組,炎調(diào)方組ig 9.9 g/kg炎調(diào)方,地塞米松組ig 0.45 mg/kg地塞米松,每天給藥1次,連續(xù)給藥3 d。末次ig 2 h后采用盲腸結(jié)扎穿孔術(shù)(CLP)制備膿毒癥ALI模型,分別于造模后24 h處死動物,進行肺組織HE染色,測定各組大鼠濕/干重比(W/D),肺組織MPO、MDA水平。結(jié)果 模型組大鼠肺組織損傷程度、MPO及MDA水平較對照組及假手術(shù)組顯著增高(P<0.01),炎調(diào)方組、地塞米松組肺組織損傷程度、MPO及MDA水平較模型組顯著降低(P<0.05、0.01)。結(jié)論 炎調(diào)方可有效減輕膿毒癥ALI大鼠的炎癥及氧化應(yīng)激反應(yīng)。

Objective To observe the influence of Yantiaofang on myeloperoxidase (MPO) and malondialdehyde (MDA) in lung tissue of rats with acute lung injury induced by sepsis. Methods Male clean SD rats were randomly divided into control group, Sham group, model group, Yantiaofang group, and dexamethasone group. Rats of Yantiaofang group and dexamethasone group were fed respectively by 9.9 g/kg and 0.45 mg/kg dosage once daily for 3 d. After 2 h of the last administration, acute lung injury induced by sepsis model was reproduced in rats with cecal ligation and puncture (CLP). Each rat was tested after the 24 h when the CLP completed. Histopathological sections of the lung tissues of rats were stained by HE for the observation of histopathological changes. The ratio of W/D and the contents of MPO and MDA in lung tissue were also measured for the observation of inflammation. Results Compared with control group and Sham operation group, there was significant increase in the ratio of W/D, MPO and MDA in rat lung tissue of model group (P<0.01). On the other side, compared with model group, the ratio of W/D, MPO and MDA in rat lung tissue of Yantiaofang group and dexamethasone group were decreased (P<0.05, 0.01). Conclusion Yantiaofang can effectively decrease the inflammation and oxidative stress in rats with acute lung injury induced by sepsis.

國家自然基金資助項目(81303105)