50值為(1.03±0.40) μmol/L。綜合分析確定DOX的受試低、高濃度分別為0.09和3.44 μmol/L,分別于藥物暴露0、6、24和48 h時收獲并測定樣品。結論 建立了一種確定體外心肌毒性代謝組學研究中受試藥物濃度及受試時間的方法,為此類體外代謝組學研究提供參考依據(jù)。;Objective To select the test concentrations and test times of model drug doxorubicin (DOX) for metabonomic study of the in vitro cardiotoxicity. Methods The cell viability of DOX on cardiomyocytes for 24, 48 and 96 h were determined by MTS assay, respectively, so as to define the optimum total test time. Afterwards, the cell viability of DOX at different concentrations on primary cardiomyocytes were determined, then the low and high concentration of test drug DOX and different test time points for metabonomic study of the in vitro cardiotoxicity were defined through observe the morphological changes of cells under microscope. Results The optimum total test time was 48 h, during which the concentration of DOX resulting in 50% viability of primary cardiomyocytes (IC50) was (1.03±0.40) μmol/L. The test low and high concentrations for metabonomic experiment of the in vitro cardiotoxicity were defined as 0.09 and 3.44 μmol/L, respectively. The cardiomyocytes samples for metabonomics were harvested and determined at 0, 6, 24 and 48 h after drug exposure, respectively. Conclusions A method of selection of test drug concentrations and test times for metabonomic study of the in vitro cardiotoxicity was established, which provide references for such in vitro metabonomic studies."/>

醉酒后少妇被疯狂内射视频,一本色道久久综合一,在线天堂新版资源www在线下载,中文字幕乱人伦高清视频,中字幕视频在线永久在线观看免费

首頁 > 過刊瀏覽>2016年第39卷第3期 >2016,39(3):398-402. DOI:10.7501/j.issn.1674-6376.2016.03.013
上一篇 | 下一篇

體外心肌毒性代謝組學研究中模型藥物多柔比星濃度及受試時間篩選

Slection of test concentrations and test times of model drugs for metabonomic studies of in vitro cardiotoxicity

發(fā)布日期:2016-05-27
您是第位訪問者
藥物評價研究 ® 2025 版權所有
技術支持:北京勤云科技發(fā)展有限公司
津備案:津ICP備13000267號 互聯(lián)網(wǎng)藥品信息服務資格證書編號:(津)-非經(jīng)營性-2015-0031