目的 研究板藍(lán)根多糖（IRPS）對機(jī)體不同免疫狀態(tài)的調(diào)節(jié)作用。方法 36只頸靜脈插管大鼠，隨機(jī)分為6組：免疫正常（NS）組、免疫亢進(jìn)（IH）組、免疫抑制（IS）組、IRPS組、IH+IRPS組、IS+IRPS組，每組6只；各組大鼠均ip雞卵清蛋白進(jìn)行免疫，應(yīng)用環(huán)磷酰胺（Cy）注射制備大鼠IH和IS模型，ig 60 mg/kg IRPS，每天給藥1次，連續(xù)給藥3 d；免疫后第6和12天，每組大鼠處死3只，測定T、B淋巴細(xì)胞增殖情況和NK細(xì)胞活性，試劑盒法檢測各組血清OVA抗體水平；免疫后第1～10、12天，頸靜脈采血，分離血清，液相芯片技術(shù)檢測γ-干擾素（IFN-γ）、白細(xì)胞介素-2（IL-2）、白細(xì)胞介素-4（IL-4）、白細(xì)胞介素-6（IL-6）、白細(xì)胞介素-10（IL-10）以及腫瘤壞死因子-α（TNF-α）水平。結(jié)果 IRPS對于不同免疫狀態(tài)大鼠的B淋巴細(xì)胞增殖、NK細(xì)胞活性均具有雙向調(diào)節(jié)作用；對Th1型細(xì)胞因子IFN-γ、TNF-α和Th2型細(xì)胞因子IL-4、IL-6、IL-10具有雙向調(diào)節(jié)作用；對免疫功能正常大鼠具有免疫增強(qiáng)作用。結(jié)論 IRPS能夠抑制IH組大鼠的亢進(jìn)趨勢，提升IS組大鼠的免疫功能，對Cy造模大鼠具有雙向免疫調(diào)節(jié)作用。

Objective To investigate the regulation of Isatidis Radix polysaccharides (IRPS) to body in different immune status. Methods Totally 36 rats fixed with juglar vein catheter were randomly divided into six groups: normal group (NS), immune hyperfunction (IH) group, immunosuppressive (IS) group, IRPS group, IH+IRPS group, and IS+IRPS group, six rats in each group. The immune hyperfunctional and immunosuppressed model rats were established by Cytoxan injection, and treated with 60 mg/kg IRPS once daily for 3 d. After 6 and 12 d of immunization, three rats were killed. Lymphocyte proliferation and NK cell activity were determined. A kit was used to detect the level of serum OVA antibody. After 1-10 and 12 d of immunization, collected jugular vein blood, separated serum, and liquid chip technology was used to detect the levels of IFN-γ, IL-4, IL-2, IL-6, IL-10, and TNF-α. Results The proliferation ability of B lymphocytes and NK cells activity in both IH and IS groups were regulated, and the secretion of Th1 cytokines IFN-γ, TNF-α and Th2 cytokines IL-4, IL-6, and IL-10 was dual-directional regulated by IRPS. The immune function of control group was enhanced by IRPS. Conclusion It is indicated that IRPS can inhibit the accentuation of immune system in hyperfunction group, and enhance the immune function of immunosuppressed rats, so we conclude that the IRPS has a dual regulation effect on immune system of model rats treated with cytoxan.

河南省基礎(chǔ)與前沿技術(shù)研究項(xiàng)目（152300410076），河南省科技攻關(guān)項(xiàng)目（152102110048），河南省重大科技專項(xiàng)（111100110300）