[關(guān)鍵詞]
[摘要]
目的 研究大黃酸(RH)配伍羥基紅花黃色素A(HSYA)對(duì)慢性腎病(CKD)模型大鼠抗炎、抗氧化、抗凋亡能力的影響及其可能機(jī)制。方法 30只雄性SD大鼠隨機(jī)分為假手術(shù)組、模型組、RH(100 mg/kg)組、HSYA(50 mg/kg)組和RH(100 mg/kg)+HSYA(50 mg/kg)組,采用單側(cè)輸尿管結(jié)扎(UUO)建立CKD模型。試劑盒法測(cè)定大鼠腎組織丙二醛(MDA)水平、超氧化物歧化酶(SOD)和谷胱甘肽過(guò)氧化物酶(GSH-Px)活性;Masson染色觀察腎組織病理變化;TUNEL染色檢測(cè)細(xì)胞凋亡情況;Western blotting檢測(cè)腎組織中IκBα、p-IκBα、NF-κB p-65、p-p65和Bcl-2、Bax蛋白表達(dá)。結(jié)果 RH和HSYA配伍使用明顯降低MDA水平,提高SOD及GSH-Px活性;減少腎小球炎細(xì)胞浸潤(rùn),改善腎小管間質(zhì)纖維化等病理改變;抑制細(xì)胞凋亡;抑制磷酸化的IκBα及p65蛋白表達(dá);使Bcl-2蛋白表達(dá)上調(diào)、Bax表達(dá)下調(diào),且效果好于單用組。結(jié)論 在CKD進(jìn)程中,RH和HSYA配伍使用可抑制腎臟組織的氧化損傷,抑制腎小球炎細(xì)胞浸潤(rùn),降低細(xì)胞凋亡,且效果好于兩個(gè)藥物單用。
[Key word]
[Abstract]
Objective To investigate the anti-inflammatory, anti-oxidant, and anti-apoptotic effects of compatibility of rhein (RH) and hydroxy saffloweryellow A (HSYA) on rat chronic kidney with unilateral ureteral obstruction (UUO) and its mechanisms. Method The model of chronic kidney disease (CKD) was induced by UUO, 30 male SD rats were divided randomly into Sham group, model group, RH (100 mg/kg) group, HSYA (50 mg/kg) group, and RH (100 mg/kg)+HSYA (50 mg/kg) group. The level of MDA, activities of SOD and GSH of kidney tissue were measured by kits. The kidney histological change was measured with Masson staining. The cell apotosis was analyzed using TUNEL staining. The expression of IκBα, NF-κB p65, p-IκBα, p-p65, Bcl-2, and Bax protein was examined by Western blotting. Results Compatibility of RH and HSYA significantly decreased the level of MDA and increase the activities of SOD and GSH-Px, reduced glomerular inflammatory cell infiltration, improved the renal tubular interstitial fibrosis and pathological change, inhibited apoptosis, inhibited the expression of p-IκBα and p-p65 protein, up-regulated expression of Bcl-2 protein, and down-regulated expression of Bax, and the effects were better than those in single group. Conclusion In the process of CKD, the compatibility of HSYA and RH can inhibit the oxidative damage of renal tissue, inhibit the infiltration of inflammatory cells and reduce apoptosis, and the effect is better than that of the two drugs alone.
[中圖分類號(hào)]
[基金項(xiàng)目]
國(guó)家自然科學(xué)基金資助項(xiàng)目(81373947;81302695)