目的 研究安絡(luò)小皮傘醇提取物（MAEE）對(duì)坐骨神經(jīng)慢性壓迫性損傷（CCI）所致神經(jīng)病理性疼痛大鼠的鎮(zhèn)痛作用并探索其作用機(jī)制。方法 40只成年SD大鼠隨機(jī)分為假手術(shù)組、模型組及MAEE高、中、低劑量（800、400、200 mg/kg）組，每組8只。CCI術(shù)后14 d，連續(xù)ig給藥7 d。于1、3、5、7 d給藥后2 h測(cè)定大鼠機(jī)械痛閾（MWT）值和熱痛閾（TWL）值，并在停藥后連續(xù)測(cè)定3 d。于給藥7 d后，取各組大鼠脊髓L₄-L₆節(jié)段，ELISA及實(shí)時(shí)熒光定量PCR（qRT-PCR）法檢測(cè)炎癥因子腫瘤壞死因子-α（TNF-α）及白細(xì)胞介素-1β（IL-1β）的表達(dá)，Western blotting法檢測(cè)MAPK家族蛋白p-ERK、p-p38、p-JNK的表達(dá)變化。結(jié)果 與模型組比較，連續(xù)7 d給予MAEE能夠劑量依賴(lài)性的緩解CCI誘導(dǎo)的大鼠機(jī)械學(xué)過(guò)敏及熱痛學(xué)超敏（P<0.05、0.01）；下調(diào)CCI大鼠脊髓L₄-L₆節(jié)段炎癥因子TNF-α和IL-1β的水平以及p-ERK、p-p38、p-JNK的蛋白表達(dá)（P<0.05、0.01）。結(jié)論 MAEE劑量依賴(lài)性的緩解CCI誘導(dǎo)的機(jī)械學(xué)超敏及熱痛學(xué)過(guò)敏，該作用可能與其抑制CCI大鼠脊髓TNF-α、IL-1β等炎性細(xì)胞因子的表達(dá)及降低MAPK磷酸化蛋白表達(dá)相關(guān)。

Objective To evaluate the analgesic effects and mechanism of Marasmius androsaceus ethanolic extract (MAEE) in chronic constriction injury induced neuropathic pain. Methods Totally 40 adult SD rats were randomly divided into Sham group, model group, high-, mid-, and low-dose (800, 400, and 200 mg/kg) MA groups. After 14 d of CCI, rats were continually treated with different doses of MAEE by ig administration for 7 d. Mechanical withdrawal thresholds (MWT) and thermal withdrawal latency (TWL) were tested. After 7 d, spinal dorsal horns (L₄-L₆) of rats were taken. Expression of TNF-α and IL-1β was determined by ELISA and qRT-PCR respectively, and the expression level of MAPK signaling way proteins was determined by Western blotting in the spinal dorsal horn (L₄-L₆) of rats. Results Compared with model group, the pain threshold increased in MA group in a dose-dependent manner by repeated administration of MA for 7 d (P<0.05?0.01). The expression of TNF-α and IL-1β decreased after MA treated, and MA can also reduce the expression of p-ERK, p-p38, and p-JNK MAPK in spinal cord of CCI rats (P<0.05, 0.01). Conclusion MA can relieve the pain in rat models of chronic constriction injury of sciatic nerve in a dose-dependent manner. The possible mechanism is that attenuating the expression of TNF-α and IL-1β and decreasing the expression level of MAPK signaling way proteins in spinal cord.

江蘇高校優(yōu)勢(shì)學(xué)科建設(shè)工程資助項(xiàng)目（PAPD）