18(250 mm×4.6 mm,5 μm),流動(dòng)相為0.1%磷酸水溶液-甲醇(35∶65),激發(fā)波長(zhǎng)為265 nm,吸收波長(zhǎng)為378 nm。結(jié)果 血漿中內(nèi)源性物質(zhì)對(duì)待測(cè)物無干擾;線性范圍為0.1~100 μg/mL,R2=0.999;定量下限為0.1 μg/mL;高中低濃度樣品提取回收率在87.76%~95.65%,批內(nèi)批間精密度RSD在0.86 %~1.87%,相對(duì)誤差RE均小于5%,符合相關(guān)生物樣品檢測(cè)標(biāo)準(zhǔn)。SD大鼠ig給予2.0 g/kg的阿齊沙坦酯鉀后,阿齊沙坦在大鼠體內(nèi)AUC0-t為(5451.94±297.96)μg/L·h,Cmax為(258.01±49.75)μg/mL。結(jié)論 建立了專屬性強(qiáng)、靈敏度高、重復(fù)性好的HPLC-熒光分析方法,可用于阿齊沙坦在大鼠血漿中血藥濃度測(cè)定。;Objective To establish a HPLC-fluorescence method for determination of concentration of Azilsartan in rat plasma. Methods Chroma-tographic column: Aglient Epilent plμs C18 (250 mm×4.6 mm, 5 μm); Mobile phase was 0.1% phosphoric acid solution and methanol (35:65); Excitation wavelength was 265 nm; Ereceiver wavelength was 378 nm. Results Endogenous substance in plasma samples had no effect on result. Calibration curves were linear over 0.1-100 μg/mL (R2=0.999) and the minimum detection concentration was 0.1 μg/mL. The recoveries of azilsartan from plasma were between 87.76% and 95.65%. The RSD values of intra and inter-day were between 0.86%-1.87%. The accuracy (RE%) was blow 5%. After i.g. of 2 000 mg/kg Azilsartan, the pharmacokinetics parameters were estimated as follows: AUC0-t values were (5 451.94±297.96) μg/L·h and Cmax were (258.01±49.75) μg/mL. Conclusion A sensitive and reliable HPLC-fluorescence method is developed, and it can be used for determination of concentration of Azilsartan in rat plasma."/>

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首頁 > 過刊瀏覽>2016年第39卷第4期 >2016,39(4):572-576. DOI:10.7501/j.issn.1674-6376.2016.04.011
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阿齊沙坦在大鼠血漿中的藥動(dòng)學(xué)研究

Determination of concentration of Azilsartan in rat plasma by HPLC with fluorescence detection

發(fā)布日期:2016-07-22
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