目的 通過UFLC-QTOF-MS技術(shù)，評(píng)價(jià)通脈顆粒的質(zhì)量。方法 對(duì)10個(gè)生產(chǎn)企業(yè)的61批次的通脈顆粒，采用Agilent proshell 120（100 mm×3.0 mm，2.7 μm），流動(dòng)相是甲醇-緩沖鹽，梯度洗脫，體積流量0.4 mL/min，柱溫40℃，ESI源，正離子模式下采集質(zhì)譜數(shù)據(jù)。應(yīng)用Markerview軟件進(jìn)行主成分分析（PCA）對(duì)通脈顆粒進(jìn)行分組。結(jié)果 正離子模式時(shí)PCA分析能很好的區(qū)分通脈顆粒的質(zhì)量，通過t檢驗(yàn)找出了大豆素8-C-芹糖（1,6）-O-葡萄糖苷和1,5-Anhydro-1-[5,7- dimethoxy-2-（4-methoxyphenyl）-4-oxo-4H-chromen-8-yl]hexitol兩個(gè)差異最大的化合物。結(jié)論 PCA分析可以作為評(píng)價(jià)通脈顆粒質(zhì)量的分類方法。

Objective To evaluate the quality of Tongmai Granule by UFLC-QTOF-MS. Methods The UFLC was performed on Agilent proshell 120 column (100 mm×3.0 mm, 2.7 μm) with the mobile phase of MeOH-buffer salt (43:57) by gradient elution, the flow rate was 0.4 mL/min, the column temperature was 40℃, electrospray ionization source was used under the positive ion mode. Tongmai Granule was classified by principle component analysis (PCA) with Markerview software. Results PCA analysis could distinguish the quality of Tongmai Granule under positive ion mode. Daidzein 8-C-apiosyl(1,6)-O-glucoside and 1,5-anhydro-1-[5,7-dimethoxy-2-(4-methoxyphenyl)-4-oxo-4H-chromen-8-yl]hexitol were found to be the biggest different compounds by t test. Conclusion PCA is an effective classification method to evaluate the quality of Tongmai Granule.

遼寧省自然科學(xué)基金優(yōu)秀人才培養(yǎng)項(xiàng)目（2015020698）