3-PPT、PPT、PT同步檢測的方法;從人參根皂苷水解產(chǎn)物中篩選發(fā)現(xiàn)具有降血糖活性的三醇組皂苷元。方法 采用HPLC-ELSD法測定25-OH-PPT、25-OCH3-PPT、PPT、PT;采用體外實驗測定4種皂苷元對α-葡萄糖苷酶的抑制作用。結果 HPLC-ELSD測定結果表明,25-OH-PPT、25-OCH3-PPT、PPT、PT在0.05~1.0 mg具有良好的線性關系,回收率分別為95.0%、98.4%、103.3%和96.0%,RSD分別為1.05%(n=4)、1.87%(n=4)、2.15%(n=4)和1.15%(n=4),質量分數(shù)分別為8.07%、4.55%、1.46%和4.53%。4種皂苷元對α-葡萄糖苷酶呈現(xiàn)出不同強度的抑制活性,25-OH-PPT抑制活性強于阿卡波糖。結論 4種皂苷元降血糖活性強弱順序為25-OH-PPT > 25-OCH3-PPT > PPT > PT。HPLC/ELSD測定方法簡單、快速、有效。;Objective To determine the inhibitory activity of rare ginsenosides on α-glucosidase, and to select hypoglycemic compounds from ginsenosides. Meanwhile, to establish a method to determinate the contents of ginsenoside 25-OH-PPT, 25-OCH3-PPT, PPT, and PT in hydrolysis products of total saponins in ginseng roots. Results The inhibitory activity of ginsenosides on α-glucosidase revealed that the inhibitory activities of 25-OH PPT, 25-OCH3-PPT, PPT, and PT were better than that of positive drug acarbose while PT was worse than acarbose. Meanwhile, HPLC-ELSD results revealed that 25-OH PPT, 25-OCH3-PPT, PPT, and PT showed good linear relationship in the region of (0.05-1.0) mg/mL. The recoveries were 95.0%, 98.4%, 103.3%, and 96.0%, respectively, RSD values were 1.05% (n=4), 1.87% (n=4), 2.15% (n=4), and 1.15% (n=4), respectively, and the content are 8.07%, 4.55%, 1.46%, and 4.53% respectively. Conclusion The order of their hypoglycemic acitivity is 25-OH-PPT > 25-OCH3-PPT > PPT > PT. Assay method of HPLC/ELSD is simple, fast, and effective."/>