目的 建立測定金線蓮中阿魏酸的定量方法，為金線蓮及其相關(guān)產(chǎn)品的質(zhì)量控制提供科學(xué)的方法。方法 采用RP-HPLC法，選用Lanbo-Kromasil-C18色譜柱；以甲醇-1%乙酸溶液（32∶68）為流動相；檢測波長321 nm，體積流量1.0 mL/min，柱溫為25℃。結(jié)果 阿魏酸在36~1 080 ng與峰面積呈良好的線性關(guān)系（r=0.999 9）。平均加樣回收率為99.7%，RSD=2.5%。結(jié)論 本法簡便、快速、準確、重復(fù)性好，可作為金線蓮質(zhì)量控制的方法。

Objective To establish a method for determination of the content of ferulic acid in Anoectochilus roxburghii. and to provide a scientific method for quality control of A. roxburghii and related products. Methods The conditions were as follows:reverse phase HPLC and Lanbo-Kromasil-C18 column, methanol-1% acetic acid solution (32:68) as mobile phase, detection wavelength of 321 nm, the volume flow rate of 1 mL/min, and column temperature of 25℃. Results Ferulic acid showed good linear relationship in the range of 36-1 080 ng (r=0.999 9). The average recovery was 99.7%, and the average rate of recovery was RSD=2.5%. Conclusion This method is simple, rapid, accurate, and reproducible, and can be used as an ideal method for the quality control of A. roxburghii.